Daniela-Saveta Popa1, Pompei Bolfa2, Bela Kiss1, Laurian Vlase3, Ramona Păltinean4, Anca Pop1, Cornel Cătoi5, Gianina Crişan3, Felicia Loghin1. 1. Department of Toxicology, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400012, Romania. 2. Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 400372, Romania; Department of Biomedical Sciences, Ross University School of Veterinary Medicine, PO Box 334, Basseterre, St. Kitts, West Indies. 3. Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, 'Iuliu Haţieganu' University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400012, Romania. 4. Department of Pharmaceutical Botany, Faculty of Pharmacy, 'Iuliu Haţieganu' University of Medicine and Pharmacy Cluj-Napoca, Cluj-Napoca 400012, Romania. 5. Department of Pathology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 400372, Romania.
Abstract
OBJECTIVE: To evaluate the influence of an extract of Genista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and 2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA). METHODS: Adult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples were taken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation and in vivo hydroxyl radical production were evaluated by histological analysis along with malondialdehyde and 2,3-dihydroxybenzoic acid detection. RESULTS: The severity of histopathological changes in liver and kidneys was lower after GT treatment than after BPA or BPA+MP treatment. A minimal thyroid receptor antagonist effect was only observed after BPA+MP treatment. The abnormal folliculogenesis increased in a time-dependent manner, and the number of corpus luteum decreased. No significant histological alterations were found in the uterus. The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-induced hydroxyl radical generation. CONCLUSION: GT L. extract ameliorates the toxic effects of BPA and is proved to have antioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.
OBJECTIVE: To evaluate the influence of an extract of Genista tinctoria L. herba (GT) or methylparaben (MP) on histopathological changes and 2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A (BPA). METHODS: Adult female Wistar rats were orally exposed for 90 d to BPA (50 mg/kg), BPA+GT (35 mg isoflavones/kg) or BPA+MP (250 mg/kg). Plasma and tissue samples were taken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation and in vivo hydroxyl radical production were evaluated by histological analysis along with malondialdehyde and 2,3-dihydroxybenzoic acid detection. RESULTS: The severity of histopathological changes in liver and kidneys was lower after GT treatment than after BPA or BPA+MP treatment. A minimal thyroid receptor antagonist effect was only observed after BPA+MP treatment. The abnormal folliculogenesis increased in a time-dependent manner, and the number of corpus luteum decreased. No significant histological alterations were found in the uterus. The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-induced hydroxyl radical generation. CONCLUSION: GT L. extract ameliorates the toxic effects of BPA and is proved to have antioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.
Authors: Amira M Aker; Deborah J Watkins; Lauren E Johns; Kelly K Ferguson; Offie P Soldin; Liza V Anzalota Del Toro; Akram N Alshawabkeh; José F Cordero; John D Meeker Journal: Environ Res Date: 2016-07-21 Impact factor: 6.498
Authors: A C Gore; V A Chappell; S E Fenton; J A Flaws; A Nadal; G S Prins; J Toppari; R T Zoeller Journal: Endocr Rev Date: 2015-11-06 Impact factor: 19.871
Authors: Marius Emil Rusu; Carmen Georgiu; Anca Pop; Andrei Mocan; Bela Kiss; Oliviu Vostinaru; Ionel Fizesan; Maria-Georgia Stefan; Ana-Maria Gheldiu; Letitia Mates; Rebeca Moldovan; Dana Maria Muntean; Felicia Loghin; Laurian Vlase; Daniela-Saveta Popa Journal: Antioxidants (Basel) Date: 2020-05-14