V S Bogdanova1, O E Kosterin, A K Yadrikhinskiy. 1. Institute of Cytology and Genetics of Siberian Division of Russian Academy of Sciences, Acad. Lavrentyev ave. 10, Novosibirsk, 630090, Russia.
Abstract
KEY MESSAGE: Divergent wild and endemic peas differ in hybrid sterility in reciprocal crosses with cultivated pea depending on alleles of a nuclear 'speciation gene' involved in nuclear-cytoplasmic compatibility. BACKGROUND: In hybrids between cultivated and wild peas, nuclear-cytoplasmic conflict frequently occurs. One of the nuclear genes involved, Scs1, was earlier mapped on Linkage Group III. RESULTS: In reciprocal crosses of seven divergent pea accessions with cultivated P. sativum, some alleles of Scs1 manifested incompatibility with an alien cytoplasm as a decrease in pollen fertility to about 50 % in the heterozygotes and lack of some genotypic classes among F2 segregants. Earlier, we defined monophyletic evolutionary lineages A, B, C and D of pea according to allelic state of three markers, from nuclear, plastid and mitochondrial genomes. All tested representatives of wild peas from the lineages A and C exhibited incompatibility due to Scs1 deleterious effects in crosses with testerlines of P. sativum subsp. sativum (the common cultivated pea) at least in one direction. A wild pea from the lineage B and a cultivated pea from the lineage D were compatible with the testerline in both directions. The tested accession of cultivated P. abyssinicum (lineage A) was partially compatible in both directions. The Scs1 alleles of some pea accessions even originating from the same geographic area were remarkably different in their compatibility with cultivated Pisum sativum cytoplasm. CONCLUSION: Variability of a gene involved in reproductive isolation is of important evolutionary role and nominate Scs1 as a speciation gene.
KEY MESSAGE: Divergent wild and endemic peas differ in hybrid sterility in reciprocal crosses with cultivated pea depending on alleles of a nuclear 'speciation gene' involved in nuclear-cytoplasmic compatibility. BACKGROUND: In hybrids between cultivated and wild peas, nuclear-cytoplasmic conflict frequently occurs. One of the nuclear genes involved, Scs1, was earlier mapped on Linkage Group III. RESULTS: In reciprocal crosses of seven divergent pea accessions with cultivated P. sativum, some alleles of Scs1 manifested incompatibility with an alien cytoplasm as a decrease in pollen fertility to about 50 % in the heterozygotes and lack of some genotypic classes among F2 segregants. Earlier, we defined monophyletic evolutionary lineages A, B, C and D of pea according to allelic state of three markers, from nuclear, plastid and mitochondrial genomes. All tested representatives of wild peas from the lineages A and C exhibited incompatibility due to Scs1 deleterious effects in crosses with testerlines of P. sativum subsp. sativum (the common cultivated pea) at least in one direction. A wild pea from the lineage B and a cultivated pea from the lineage D were compatible with the testerline in both directions. The tested accession of cultivated P. abyssinicum (lineage A) was partially compatible in both directions. The Scs1 alleles of some pea accessions even originating from the same geographic area were remarkably different in their compatibility with cultivated Pisum sativum cytoplasm. CONCLUSION: Variability of a gene involved in reproductive isolation is of important evolutionary role and nominate Scs1 as a speciation gene.
Authors: Vera S Bogdanova; Olga O Zaytseva; Anatoliy V Mglinets; Natalia V Shatskaya; Oleg E Kosterin; Gennadiy V Vasiliev Journal: PLoS One Date: 2015-03-19 Impact factor: 3.240
Authors: Jyoti Devi; Gyan P Mishra; Vidya Sagar; Vineet Kaswan; Rakesh K Dubey; Prabhakar M Singh; Shyam K Sharma; Tusar K Behera Journal: Genes (Basel) Date: 2022-02-08 Impact factor: 4.096