X Tan1, S Sun1, Y Liu2, T Zhu1, K Wang3, T Ren1, Z Wu1, H Xu1, L Zhu4. 1. Department of Ophthalmology, Wuxi No. 2, People's Hospital, Nanjing Medical University, Wuxi, China. 2. Department of Cataract, Zhongshan Ophthalmic Centre, Guangzhou, China. 3. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, China. 4. Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
Abstract
PURPOSE: To determine the levels of Th17-associated cytokines, particularly interleukin (IL)-17 and IL-22 in tears of patients with dry eye syndrome. METHODS: Tear samples were collected from 20 healthy volunteers, 20 dry eye (DE) patients with non-Sjögren's syndrome (NSSDE) and 20 DE patients with Sjögren's syndrome (SSDE). Symptom questionnaire was self-administered and multiple dry eye disease (DED)-related clinical tests were performed. The levels of IL-17 and IL-22 in tears were measured by enzyme-linked immunosorbent assay. RESULTS: The levels of IL-17 and IL-22 were significantly increased in tears of DE patients compared with those of controls and also higher in SSDE patients compared with those of NSSDE patients (P<0.05). Moreover, the levels of IL-17 and IL-22 were positively correlated with questionnaire score and keratopathy score but negatively correlated with tear film break-up time and Schirmer I test in both NSSDE and SSDE patients (P<0.05). CONCLUSIONS: The levels of IL-17 and IL-22 in tears were significantly increased in DE patients, which were associated with the disease severity. Therefore, Th17 cell-associated cytokines, particularly IL-17 and IL-22, may have important roles in the immunopathogenesis of the DED.
PURPOSE: To determine the levels of Th17-associated cytokines, particularly interleukin (IL)-17 and IL-22 in tears of patients with dry eye syndrome. METHODS: Tear samples were collected from 20 healthy volunteers, 20 dry eye (DE) patients with non-Sjögren's syndrome (NSSDE) and 20 DE patients with Sjögren's syndrome (SSDE). Symptom questionnaire was self-administered and multiple dry eye disease (DED)-related clinical tests were performed. The levels of IL-17 and IL-22 in tears were measured by enzyme-linked immunosorbent assay. RESULTS: The levels of IL-17 and IL-22 were significantly increased in tears of DE patients compared with those of controls and also higher in SSDE patients compared with those of NSSDE patients (P<0.05). Moreover, the levels of IL-17 and IL-22 were positively correlated with questionnaire score and keratopathy score but negatively correlated with tear film break-up time and Schirmer I test in both NSSDE and SSDE patients (P<0.05). CONCLUSIONS: The levels of IL-17 and IL-22 in tears were significantly increased in DE patients, which were associated with the disease severity. Therefore, Th17 cell-associated cytokines, particularly IL-17 and IL-22, may have important roles in the immunopathogenesis of the DED.
Authors: Lihui Luo; De-Quan Li; Amish Doshi; William Farley; Rosa M Corrales; Stephen C Pflugfelder Journal: Invest Ophthalmol Vis Sci Date: 2004-12 Impact factor: 4.799
Authors: Nancy J Reyes; Chen Yu; Rose Mathew; Carolina M Kunnen; Joan Kalnitsky; Rachel L Redfern; Andrea Leonardi; Victor L Perez; Amanda S MacLeod; Preeya K Gupta; Daniel R Saban Journal: Sci Transl Med Date: 2018-07-25 Impact factor: 17.956