OBJECTIVE: This prospective study was set up in order to analyze whether additional treatment (cutting off supernumerous cumulus cells; adding hyaluronidase) of the cumulus-oocyte-complex (COC) would help to improve treatment outcome. MATERIAL AND METHODS: COCs from 50 patients were prospectively subdivided into a control group A (no manipulation of COC) and two study groups. In group B, surplus cumulus cells were cut off using syringes, and in the second study group COCs were incubated with a 1:11 dilution of hyaluronidase (final concentration 7 IU/l). Main outcome measures were fertilization rate, embryo development, as well as rates of implantation, pregnancy, and live birth. RESULTS: Fertilization was higher in group C as compared to the untreated control group A (p<0.05). However, complete fertilization failure could not be avoided by any of the modified IVF approaches. Compaction on day 4 and blastocyst quality on day 5 were significantly improved in group C as compared to group B (but not to group A). Rates of implantation, pregnancy, and live birth were not affected by any of the methods. CONCLUSION: ICSI seems to be the only choice for avoiding the vast majority of fertilization failures after IVF.
OBJECTIVE: This prospective study was set up in order to analyze whether additional treatment (cutting off supernumerous cumulus cells; adding hyaluronidase) of the cumulus-oocyte-complex (COC) would help to improve treatment outcome. MATERIAL AND METHODS: COCs from 50 patients were prospectively subdivided into a control group A (no manipulation of COC) and two study groups. In group B, surplus cumulus cells were cut off using syringes, and in the second study group COCs were incubated with a 1:11 dilution of hyaluronidase (final concentration 7 IU/l). Main outcome measures were fertilization rate, embryo development, as well as rates of implantation, pregnancy, and live birth. RESULTS: Fertilization was higher in group C as compared to the untreated control group A (p<0.05). However, complete fertilization failure could not be avoided by any of the modified IVF approaches. Compaction on day 4 and blastocyst quality on day 5 were significantly improved in group C as compared to group B (but not to group A). Rates of implantation, pregnancy, and live birth were not affected by any of the methods. CONCLUSION: ICSI seems to be the only choice for avoiding the vast majority of fertilization failures after IVF.
Authors: D G Hammitt; C H Syrop; B J Van Voorhis; D L Walker; T M Miller; K M Barud; C C Hood Journal: J Assist Reprod Genet Date: 1992-10 Impact factor: 3.412
Authors: S Oehninger; T F Kruger; T Simon; D Jones; J Mayer; S Lanzendorf; J P Toner; S J Muasher Journal: Hum Reprod Date: 1996-05 Impact factor: 6.918
Authors: Shlomit Kenigsberg; Patricia D A Lima; Leila Maghen; Brandon A Wyse; Chantal Lackan; Annie N Y Cheung; Benjamin K Tsang; Clifford L Librach Journal: PLoS One Date: 2017-04-13 Impact factor: 3.240