Literature DB >> 24584192

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

Bin Shen1, Wensheng Zhang2, Jun Zhang1, Jiankui Zhou3, Jianying Wang3, Li Chen3, Lu Wang4, Alex Hodgkins5, Vivek Iyer5, Xingxu Huang3, William C Skarnes5.   

Abstract

Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.

Entities:  

Mesh：

Substances：

Year:  2014        PMID： 24584192     DOI： 10.1038/nmeth.2857

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  26 in total

Review 1.  RNA-guided genetic silencing systems in bacteria and archaea.

Authors:  Blake Wiedenheft; Samuel H Sternberg; Jennifer A Doudna
Journal:  Nature       Date:  2012-02-15       Impact factor: 49.962

Review 2.  Genome editing with engineered zinc finger nucleases.

Authors:  Fyodor D Urnov; Edward J Rebar; Michael C Holmes; H Steve Zhang; Philip D Gregory
Journal:  Nat Rev Genet       Date:  2010-09       Impact factor: 53.242

3.  Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

Authors:  F Ann Ran; Patrick D Hsu; Chie-Yu Lin; Jonathan S Gootenberg; Silvana Konermann; Alexandro E Trevino; David A Scott; Azusa Inoue; Shogo Matoba; Yi Zhang; Feng Zhang
Journal:  Cell       Date:  2013-08-29       Impact factor: 41.582

4.  Knockout rats generated by embryo microinjection of TALENs.

Authors:  Laurent Tesson; Claire Usal; Séverine Ménoret; Elo Leung; Brett J Niles; Séverine Remy; Yolanda Santiago; Anna I Vincent; Xiangdong Meng; Lei Zhang; Philip D Gregory; Ignacio Anegon; Gregory J Cost
Journal:  Nat Biotechnol       Date:  2011-08-05       Impact factor: 54.908

5.  Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.

Authors:  Seung Woo Cho; Sojung Kim; Jong Min Kim; Jin-Soo Kim
Journal:  Nat Biotechnol       Date:  2013-01-29       Impact factor: 54.908

6.  Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.

Authors:  Bin Shen; Jun Zhang; Hongya Wu; Jianying Wang; Ke Ma; Zheng Li; Xueguang Zhang; Pumin Zhang; Xingxu Huang
Journal:  Cell Res       Date:  2013-04-02       Impact factor: 25.617

7.  Megabase chromatin domains involved in DNA double-strand breaks in vivo.

Authors:  E P Rogakou; C Boon; C Redon; W M Bonner
Journal:  J Cell Biol       Date:  1999-09-06       Impact factor: 10.539

8.  CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.

Authors:  Prashant Mali; John Aach; P Benjamin Stranges; Kevin M Esvelt; Mark Moosburner; Sriram Kosuri; Luhan Yang; George M Church
Journal:  Nat Biotechnol       Date:  2013-08-01       Impact factor: 54.908

9.  RNA-programmed genome editing in human cells.

Authors:  Martin Jinek; Alexandra East; Aaron Cheng; Steven Lin; Enbo Ma; Jennifer Doudna
Journal:  Elife       Date:  2013-01-29       Impact factor: 8.140

10.  RNA-guided editing of bacterial genomes using CRISPR-Cas systems.

Authors:  Wenyan Jiang; David Bikard; David Cox; Feng Zhang; Luciano A Marraffini
Journal:  Nat Biotechnol       Date:  2013-01-29       Impact factor: 54.908
View more
  330 in total

Review 1.  Combining CRISPR/Cas9 and rAAV Templates for Efficient Gene Editing.

Authors:  Manuel Kaulich; Steven F Dowdy
Journal:  Nucleic Acid Ther       Date:  2015-11-05       Impact factor: 5.486

Review 2.  Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery.

Authors:  Mehmet Fatih Bolukbasi; Ankit Gupta; Scot A Wolfe
Journal:  Nat Methods       Date:  2016-01       Impact factor: 28.547

Review 3.  Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts.

Authors:  Jun Wu; Juan Carlos Izpisua Belmonte
Journal:  Transgenic Res       Date:  2016-01-28       Impact factor: 2.788

Review 4.  Gene-edited CRISPy Critters for alcohol research.

Authors:  Gregg E Homanics
Journal:  Alcohol       Date:  2018-03-07       Impact factor: 2.405

Review 5.  Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities.

Authors:  Ling Li; Shuo Hu; Xiaoyuan Chen
Journal:  Biomaterials       Date:  2018-04-18       Impact factor: 12.479

6.  Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system.

Authors:  Shenglan Li; Haipeng Xue; Bo Long; Li Sun; Tai Truong; Ying Liu
Journal:  J Vis Exp       Date:  2015-05-28       Impact factor: 1.355

7.  The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.

Authors:  Yun Xia; Ignacio Sancho-Martinez; Emmanuel Nivet; Concepcion Rodriguez Esteban; Josep Maria Campistol; Juan Carlos Izpisua Belmonte
Journal:  Nat Protoc       Date:  2014-10-23       Impact factor: 13.491

8.  Minimizing off-target effects in CRISPR-Cas9 genome editing.

Authors:  Shi-Jie Chen
Journal:  Cell Biol Toxicol       Date:  2019-07-17       Impact factor: 6.691

Review 9.  The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.

Authors:  Davide Seruggia; Lluis Montoliu
Journal:  Transgenic Res       Date:  2014-08-06       Impact factor: 2.788

10.  Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.

Authors:  Maximilian Müller; Ciaran M Lee; Giedrius Gasiunas; Timothy H Davis; Thomas J Cradick; Virginijus Siksnys; Gang Bao; Toni Cathomen; Claudio Mussolino
Journal:  Mol Ther       Date:  2015-12-14       Impact factor: 11.454
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.