| Literature DB >> 24577012 |
A Gomez-Lopez1, A Forastiero, E Cendejas-Bueno, L Gregson, E Mellado, S J Howard, J L Livermore, W W Hope, M Cuenca-Estrella.
Abstract
The impact of different mutations in the Aspergillus fumigatus ergosterol biosynthesis pathway on pathogenesis has been evaluated using a simple invertebrate mini host, the caterpillar Galleria mellonella. A set of strains that includes clinical isolates and isogenic mutants with mutations at the cyp51A gene conferring azole resistance were studied. All strains demonstrated a similar in vitro growth pattern and are equally virulent against the insect larvae. These results suggest that in A. fumigatus acquisition of this particular azole-resistance mechanism would not imply any significant change in virulence. G. mellonella may provide a convenient and inexpensive model for the in vivo prescreening of mutants of A. fumigatus, contributing to the generation of a hypotheses that can be further tested in refined experiments in mammalian models.Entities:
Keywords: cyp51A gene; isogenic mutants; nonmammalian model
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Year: 2014 PMID： 24577012 DOI： 10.1093/mmy/myt022
Source DB: PubMed Journal: Med Mycol ISSN： 1369-3786 Impact factor: 4.076