Pseudomonas hunanensis sp. nov., isolated from soil subjected to long-term manganese pollution.

A Gram-negative, polar flagella, rod-shaped bacterium LV (T) was isolated from a soil sample subjected to long-term manganese pollution in Hunan Province, China. Cells grow optimally on Luria-Bertani agar medium at 30 °C in the presence of 0-5.0 % (w/v) NaCl and pH 7-8. 16S rRNA gene sequence analysis revealed that strain LV (T) belonged to the genus Pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to Pseudomonas monteilii BCRC 17520 (T) , Pseudomonas putida BCRC 10459 (T) , Pseudomonas plecoglossicida BCRC 17517 (T) , and Pseudomonas asplenii BCRC 17131 (T) , respectively. The level of DNA-DNA relatedness between the five strains was <30 %. The DNA G+C content of strain LV (T) is 68.8 mol%. Chemotaxonomic data revealed that the strain LV(T) possesses ubiquinone Q-9. The polar lipid profile of strain LV (T) contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, and phosphatidylethanolamine. The major cellular fatty acids present are C10:03-OH (12.33 %), C16:0 (23.99 %), summed feature 3(C16:1ω7c and/or C16:1ω6c), and summed feature 8(C18:1 ω7c and C18:1 ω6c). Based on the genotypic, chemotaxonomic and phenotypic data, strain LV (T) is distinguishable from related members of the genus Pseudomonas. Thus, strain LV (T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas hunanensis sp. nov. is proposed. The type strain is LV (T) (=CICC 10558(T) = NCCB 100446(T)).