Literature DB >> 24557909

RNA-guided genome editing of mammalian cells.

Neena K Pyzocha1, F Ann Ran, Patrick D Hsu, Feng Zhang.   

Abstract

The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.

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Year:  2014        PMID： 24557909     DOI： 10.1007/978-1-62703-761-7_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  23 in total

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6.  A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines.

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7.  Definitive localization of intracellular proteins: Novel approach using CRISPR-Cas9 genome editing, with glucose 6-phosphate dehydrogenase as a model.

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8.  MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

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Journal:  Nat Protoc       Date:  2015-12-17       Impact factor: 13.491

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Review 10.  Genome-edited human stem cell-derived beta cells: a powerful tool for drilling down on type 2 diabetes GWAS biology.

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Journal:  F1000Res       Date:  2016-07-15
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