Literature DB >> 2453299

LEU2 directed expression of beta-galactosidase activity and phleomycin resistance in Yarrowia lipolytica.

C Gaillardin1, A M Ribet.   

Abstract

The nucleotide sequence of a 968 bp DNA fragment spanning the promoter and the 5' upstream sequence of the LEU2 coding sequence of the yeast Yarrowia lipolytica has been determined. A LEU2::lacZ fusion has been constructed and expressed in transformed yeast cells, showing that as few as 232 bp of the LEU2 promotor were sufficient to direct gene expression. In order to develop new markers for transformation of this yeast, the LEU2 initiation codon was destroyed by in vitro mutagenesis and replaced by a cloning site. A gene conferring phleomycin resistance in E. coli was attached to the LEU2 promoter and shown to be efficiently expressed in yeast: direct selection of phleomycin resistant transformants was possible.

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Year:  1987        PMID： 2453299     DOI： 10.1007/bf00378179

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  22 in total

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Journal:  Gene       Date:  1979-05       Impact factor: 3.688

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Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

3.  A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.

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Journal:  Cell       Date:  1983-01       Impact factor: 41.582

4.  The ovalbumin gene-sequence of putative control regions.

Authors:  C Benoist; K O'Hare; R Breathnach; P Chambon
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

5.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

6.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

7.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Expression of a transposable antibiotic resistance element in Saccharomyces.

Authors:  A Jimenez; J Davies
Journal:  Nature       Date:  1980-10-30       Impact factor: 49.962

9.  Oxygen transfer from bleomycin-metal complexes.

Authors:  N Murugesan; G M Ehrenfeld; S M Hecht
Journal:  J Biol Chem       Date:  1982-08-10       Impact factor: 5.157

10.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469
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  23 in total

1.  Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica.

Authors:  H J Wang; M T Le Dall; Y Wach; C Laroche; J M Belin; C Gaillardin; J M Nicaud
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica.

Authors:  J C Torres-Guzmán; A Domínguez
Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

3.  Cloning of the mating-type gene MATA of the yeast Yarrowia lipolytica.

Authors:  C Kurischko; P Fournier; M Chasles; H Weber; C Gaillardin
Journal:  Mol Gen Genet       Date:  1992-04

4.  Involvement of hexokinase Hxk1 in glucose catabolite repression of LIP2 encoding extracellular lipase in the yeast Yarrowia lipolytica.

Authors:  Patrick Fickers; Jean Marc Nicaud; Jacqueline Destain; Philippe Thonart
Journal:  Curr Microbiol       Date:  2005-03-15       Impact factor: 2.188

5.  Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast.

Authors:  E V Trubnikova; E P Isakova; Yu I Deryabina; A V Nikolaev
Journal:  Dokl Biochem Biophys       Date:  2016-07-15       Impact factor: 0.788

6.  Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica.

Authors:  S Matoba; J Fukayama; R A Wing; D M Ogrydziak
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

7.  Transformation of Cochliobolus lunatus with pUT 720 changes the steroid hydroxylating ability of the fungus.

Authors:  D Rozman; R Komel
Journal:  Curr Genet       Date:  1992-08       Impact factor: 3.886

8.  LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast.

Authors:  J M Beckerich; M Lambert; C Gaillardin
Journal:  Curr Genet       Date:  1994-01       Impact factor: 3.886

9.  The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.

Authors:  M Le Dall; J Nicaud; B Y Tréton; C M Gaillardin
Journal:  Curr Genet       Date:  1996-04       Impact factor: 3.886

10.  Cloning of the HIS3 gene of Yarrowia lipolytica.

Authors:  C Prodromou; I P Wright; I H Evans; E A Bevan
Journal:  Antonie Van Leeuwenhoek       Date:  1991-08       Impact factor: 2.271
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