Literature DB >> 27417717

Modified gene lacZ encoding β-galactosidase as efficient transcriptional reporter for the Yarrowia lipolytica yeast.

E V Trubnikova1, E P Isakova2, Yu I Deryabina3, A V Nikolaev4.   

Abstract

A modified variant of the β-galactosidase gene from Escherichia coli (lacZ) has been obtained. This was designed for building transcriptional reporter constructs, the product of which possesses a higher proteolytic resistance in the Yarrowia lipolytica yeasts. The study of the activity of mitochondrial voltagedependent porin (voltage-dependent anion channel, VDAC) showed a high efficiency of the reporter system based on the designed lacZ reporter compared to the wild-type lacZ gene.

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Year:  2016        PMID: 27417717     DOI: 10.1134/S160767291603008X

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN: 1607-6729            Impact factor:   0.788


  5 in total

1.  LEU2 directed expression of beta-galactosidase activity and phleomycin resistance in Yarrowia lipolytica.

Authors:  C Gaillardin; A M Ribet
Journal:  Curr Genet       Date:  1987       Impact factor: 3.886

2.  Vectors for gene expression and amplification in the yeast Yarrowia lipolytica.

Authors:  T Juretzek; M Le Dall; S Mauersberger; C Gaillardin; G Barth; J Nicaud
Journal:  Yeast       Date:  2001-01-30       Impact factor: 3.239

3.  Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation.

Authors:  K J Wiechelman; R D Braun; J D Fitzpatrick
Journal:  Anal Biochem       Date:  1988-11-15       Impact factor: 3.365

4.  A simple method for the identification and assay of extracellular plant beta-galactosidase.

Authors:  J Stano; K Mićieta; K Neubert; M Luckner; H Adam
Journal:  Pharmazie       Date:  2002-03       Impact factor: 1.267

  5 in total

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