Fatmah A Matough1, Siti B Budin2, Zariyantey A Hamid2, Mariati Abdul-Rahman3, Nasar Al-Wahaibi4, Jamaludine Mohammed2. 1. Department of Biology, Faculty of Science, Sabha University, Sabha, Libya; 2. Programme of Biomedical Sciences, School of Diagnostic Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia; 3. Department of Clinical Oral Biology, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malayasia; 4. Department of Pathology, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, Oman.
Abstract
OBJECTIVES: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabetic rats. METHODS: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. RESULTS: FBG levels for the STZ-induced diabetic rats were significantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also significantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabetic rats when compared to the control group and diabetic group with TRF. The results of the superoxide dismutase and glutathione peroxidase activities were significantly lower in the STZ-induced diabetic rats than in the normal group (P <0.001). The levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were significantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabetic rats treated with TRF. CONCLUSION: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabetic rats.
OBJECTIVES: This study was carried out to determine the effects of tocotrienol-rich fraction (TRF) (200 mg/Kg) on biomarkers of oxidative stress on erythrocyte membranes and leukocyte deoxyribonucleic acid (DNA) damage in streptozotocin (STZ)-induced diabeticrats. METHODS: Male rats (n = 40) were divided randomly into four groups of 10: a normal group; a normal group with TRF; a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, fasting blood glucose (FBG) levels, oxidative stress markers and the antioxidant status of the erythrocytes were measured. RESULTS: FBG levels for the STZ-induced diabeticrats were significantly increased (P <0.001) when compared to the normal group and erythrocyte malondialdehyde levels were also significantly higher (P <0.0001) in this group. Decreased levels of reduced glutathione and increased levels of oxidised glutathione (P <0.001) were observed in STZ-induced diabeticrats when compared to the control group and diabetic group with TRF. The results of the superoxide dismutase and glutathione peroxidase activities were significantly lower in the STZ-induced diabeticrats than in the normal group (P <0.001). The levels of DNA damage, measured by the tail length and tail moment of the leukocyte, were significantly higher in STZ-induced diabetic (P <0.0001). TRF supplementation managed to normalise the level of DNA damage in diabeticrats treated with TRF. CONCLUSION: Daily supplementation with 200 mg/Kg of TRF for four weeks was found to reduce levels of oxidative stress markers by inhibiting lipid peroxidation and increasing the levels of antioxidant status in a prevention trial for STZ-induced diabeticrats.
Authors: Jan Frank; Xiao Wei Dawn Chin; Charlotte Schrader; Gunter P Eckert; Gerald Rimbach Journal: Ageing Res Rev Date: 2011-07-07 Impact factor: 10.895
Authors: Mohd Danial Mohd Efendy Goon; Nur Izzati Zulkanain; Siti Hamimah Sheikh Abdul Kadir; Sharaniza Ab Rahim; Musalmah Mazlan; Normala Abd Latip; Mardiana Abdul Aziz; Norizal Mohd Noor Journal: Transl Gastroenterol Hepatol Date: 2022-01-25