Isolation of protoplasts from cereal leaves.

Mature leaves of Secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (Meicelase) and pectinase (Pectinol R10) produced quantities of protoplasts. Under the same conditions leaves of Triticum aestivum, Hordeum vulgare and Avena sativa also produce protoplasts but in lower yields. The wheat and rye protoplasts in culture appear to regenerate a cell wall but only a very small proportion undergo cell division.