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Literature DB >> 24248249

An evaluation of Ficoll density gradient centrifugation as a method for eliminating microbial contamination and purifying plant protoplasts.

Abstract

Ficoll density gradient centrifugation was used to generate sterile protoplasts from non-sterile Pteridium cultures. The method was found to drastically reduce levels of fungal and bacterial contamination introduced prior to centrifugation whilst concentrating and purifying protoplasts.

Year: 1986 PMID： 24248249 DOI： 10.1007/BF00269824

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

12 in total

1. Isolation of amyloplasts from developing maize endosperm.

Authors: E Echeverria; C Boyer; K C Liu; J Shannon
Journal: Plant Physiol Date: 1985-03 Impact factor: 8.340

2. An improved procedure for the isolation and purification of protoplasts from carrot suspension culture.

Authors: A R Slabas; A J Powell; C W Lloyd
Journal: Planta Date: 1980-01 Impact factor: 4.116

3. Use of fungicides in plant tissue culture.

Authors: R Shields; S J Robinson; P A Anslow
Journal: Plant Cell Rep Date: 1984-02 Impact factor: 4.570

4. The toxicity of antibiotics to plant cell cultures.

Authors: K Pollock; D G Barfield; R Shields
Journal: Plant Cell Rep Date: 1983-02 Impact factor: 4.570

5. Purification and viability determinations of plant protoplasts.

Authors: P J Larkin
Journal: Planta Date: 1976-01 Impact factor: 4.116

6. Benomyl: A broad spectrum fungicide for use in plant cell and protoplast culture.

Authors: R M Hauptmann; J M Widholm; J D Paxton
Journal: Plant Cell Rep Date: 1985-05 Impact factor: 4.570

7. Isolation of protoplasts from cereal leaves.

Authors: P K Evans; A G Keates; E C Cocking
Journal: Planta Date: 1972-06 Impact factor: 4.116

8. Intracellular Localization of Some Key Enzymes of Crassulacean Acid Metabolism in Sedum praealtum.

Authors: M H Spalding; M R Schmitt; S B Ku; G E Edwards
Journal: Plant Physiol Date: 1979-04 Impact factor: 8.340

9. Photosynthesis in Isolated Chloroplasts of the Crassulacean Acid Metabolism Plant Sedum praealtum.

Authors: M H Spalding; G E Edwards
Journal: Plant Physiol Date: 1980-06 Impact factor: 8.340

10. Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system.

Authors: R Kanai; G E Edwards
Journal: Plant Physiol Date: 1973-11 Impact factor: 8.340

6 in total

1. Elimination and molecular identification of endophytic bacterial contaminants during in vitro propagation of Bambusa balcooa.

Authors: Syandan Sinha Ray; Md Nasim Ali; Shibasis Mukherjee; Gautam Chatterjee; Maitreyi Banerjee
Journal: World J Microbiol Biotechnol Date: 2017-01-06 Impact factor: 3.312

2. Ubiquitous presence of fastidious endophytic bacteria in field shoots and index-negative apparently clean shoot-tip cultures of papaya.

Authors: Pious Thomas; Sima Kumari; Ganiga K Swarna; Devalakere P Prakash; Makki R Dinesh
Journal: Plant Cell Rep Date: 2007-05-11 Impact factor: 4.570

6. Regeneration of somatic embryos from protoplasts isolated from an embryogenic suspension culture of white spruce (Picea glauca).

Authors: S M Attree; F Bekkaoui; D I Dunstan; L C Fowke
Journal: Plant Cell Rep Date: 1987-12 Impact factor: 4.570