Literature DB >> 24430749

Purification and viability determinations of plant protoplasts.

P J Larkin1.   

Abstract

A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.

Entities:  

Year:  1976        PMID： 24430749     DOI： 10.1007/BF00393231

Source DB:  PubMed          Journal:  Planta        ISSN： 0032-0935            Impact factor:   4.116


  20 in total

1.  SEPARATION OF WHITE BLOOD CELLS.

Authors:  A BOYUM
Journal:  Nature       Date:  1964-11-21       Impact factor: 49.962

2.  The isolation of barley-aleurone protoplasts.

Authors:  L Taiz; R L Jones
Journal:  Planta       Date:  1971-06       Impact factor: 4.116

3.  Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae.

Authors:  L Cercek; B Cercek
Journal:  Biophysik       Date:  1973

4.  Serology for automated cytotoxicity assays. Contrast fluorescence test.

Authors:  J L Martel; S Jaramillo; F H Allen; P Rubinstein
Journal:  Vox Sang       Date:  1974       Impact factor: 2.144

5.  Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate.

Authors:  J Heslop-Harrison; Y Heslop-Harrison
Journal:  Stain Technol       Date:  1970-05

6.  Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells.

Authors:  L Cercek; B Cercek; C H Ockey
Journal:  Biophysik       Date:  1973

7.  Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

Authors:  B Rotman; B W Papermaster
Journal:  Proc Natl Acad Sci U S A       Date:  1966-01       Impact factor: 11.205

8.  Isolation of protoplasts from cereal leaves.

Authors:  P K Evans; A G Keates; E C Cocking
Journal:  Planta       Date:  1972-06       Impact factor: 4.116

9.  Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system.

Authors:  R Kanai; G E Edwards
Journal:  Plant Physiol       Date:  1973-11       Impact factor: 8.340

10.  Isolation and Regeneration of Tobacco Mesophyll Cell Protoplasts under Low Osmotic Conditions.

Authors:  J F Shepard; R E Totten
Journal:  Plant Physiol       Date:  1975-04       Impact factor: 8.340
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  68 in total

1.  Studies on calcium release and H2O2 level produced by the elicitor induced plant cell by fluorescence probing.

Authors:  Quan Gan; Bin Jia; Xiao Liu; Yizhu Zhang; Manxi Liu
Journal:  J Fluoresc       Date:  2011-11-11       Impact factor: 2.217

2.  Somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye ( Secale cereale L.).

Authors:  R Ma; Y-D Guo; S Pulli
Journal:  Plant Cell Rep       Date:  2003-08-29       Impact factor: 4.570

3.  Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses.

Authors:  P Genschik; Y Parmentier; A Durr; J Marbach; M C Criqui; E Jamet; J Fleck
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

4.  Cell wall arabinan is essential for guard cell function.

Authors:  Louise Jones; Jennifer L Milne; David Ashford; Simon J McQueen-Mason
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-16       Impact factor: 11.205

5.  Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici.

Authors:  Shiu-Cheung Lung; Makoto Yanagisawa; Simon D X Chuong
Journal:  Plant Cell Rep       Date:  2010-11-20       Impact factor: 4.570

6.  Heterologous expression of vacuolar H(+)-PPase enhances the electrochemical gradient across the vacuolar membrane and improves tobacco cell salt tolerance.

Authors:  Xiao-Guang Duan; Ai-Fang Yang; Feng Gao; Shang-Li Zhang; Ju-Ren Zhang
Journal:  Protoplasma       Date:  2007-12-19       Impact factor: 3.356

7.  Liposome-mediated delivery of tobacco mosaic virus RNA into petunia protoplast : Improved conditions for liposome-protoplast incubations.

Authors:  R T Fraley
Journal:  Plant Mol Biol       Date:  1983-01       Impact factor: 4.076

8.  Changes in protein synthesis during the initial stage of life of tobacco protoplasts.

Authors:  J Fleck; A Durr; M C Lett; L Hirth
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

9.  Preparation of megabase-sized tomato DNA and separation of large restriction fragments by field inversion gel electrophoresis (FIGE).

Authors:  R A van Daelen; J J Jonkers; P Zabel
Journal:  Plant Mol Biol       Date:  1989-03       Impact factor: 4.076

10.  SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa.

Authors:  Ying-Chung Lin; Wei Li; Ying-Hsuan Sun; Sapna Kumari; Hairong Wei; Quanzi Li; Sermsawat Tunlaya-Anukit; Ronald R Sederoff; Vincent L Chiang
Journal:  Plant Cell       Date:  2013-11-26       Impact factor: 11.277
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