Literature DB >> 24478405

Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures.

Nicasio Mancini1, Laura Infurnari, Nadia Ghidoli, Grazia Valzano, Nicola Clementi, Roberto Burioni, Massimo Clementi.   

Abstract

We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.

Entities:  

Mesh：

Year:  2014        PMID： 24478405      PMCID： PMC3993469          DOI： 10.1128/JCM.00142-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  26 in total

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Authors:  Kevin Alby; Lindsay M Daniels; David J Weber; Melissa B Miller
Journal:  J Clin Microbiol       Date:  2013-08-28       Impact factor: 5.948

4.  Molecular diagnosis of sepsis in neutropenic patients with haematological malignancies.

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Journal:  J Med Microbiol       Date:  2008-05       Impact factor: 2.472

5.  Use of a multiplex polymerase chain reaction system for enhanced bloodstream pathogen detection in thoracic transplantation.

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6.  Evaluation of the Verigene Gram-positive blood culture nucleic acid test for rapid detection of bacteria and resistance determinants.

Authors:  Christina M Wojewoda; Linda Sercia; Maria Navas; Marion Tuohy; Deborah Wilson; Geraldine S Hall; Gary W Procop; Sandra S Richter
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

7.  Rapid detection of Gram-positive organisms by use of the Verigene Gram-positive blood culture nucleic acid test and the BacT/Alert Pediatric FAN system in a multicenter pediatric evaluation.

Authors:  K V Sullivan; N N Turner; S S Roundtree; S Young; C A Brock-Haag; D Lacey; S Abuzaid; D L Blecker-Shelly; C D Doern
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8.  Evaluation of a microarray-based assay for rapid identification of Gram-positive organisms and resistance markers in positive blood cultures.

Authors:  Linoj P Samuel; Robert J Tibbetts; Adam Agotesku; Margaret Fey; Rhonda Hensley; Frederick A Meier
Journal:  J Clin Microbiol       Date:  2013-01-30       Impact factor: 5.948

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Journal:  PLoS Med       Date:  2013-07-02       Impact factor: 11.069
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  25 in total

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Journal:  J Clin Microbiol       Date:  2015-08-19       Impact factor: 5.948

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4.  Rapid testing using the Verigene Gram-negative blood culture nucleic acid test in combination with antimicrobial stewardship intervention against Gram-negative bacteremia.

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5.  Gut Colonization Preceding Mucosal Barrier Injury Bloodstream Infection in Pediatric Hematopoietic Stem Cell Transplantation Recipients.

Authors:  Matthew S Kelly; Doyle V Ward; Christopher J Severyn; Mehreen Arshad; Sarah M Heston; Kirsten Jenkins; Paul L Martin; Lauren McGill; Andre Stokhuyzen; Shakti K Bhattarai; Vanni Bucci; Patrick C Seed
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6.  Evaluation of the Accelerate Pheno System for Fast Identification and Antimicrobial Susceptibility Testing from Positive Blood Cultures in Bloodstream Infections Caused by Gram-Negative Pathogens.

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7.  Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay.

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Journal:  J Clin Microbiol       Date:  2015-05-20       Impact factor: 5.948

8.  Evaluation of FilmArray and Verigene systems for rapid identification of positive blood cultures.

Authors:  M M Bhatti; S Boonlayangoor; K G Beavis; V Tesic
Journal:  J Clin Microbiol       Date:  2014-07-16       Impact factor: 5.948

9.  Evaluation of the nanosphere Verigene BC-GN assay for direct identification of gram-negative bacilli and antibiotic resistance markers from positive blood cultures and potential impact for more-rapid antibiotic interventions.

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10.  Performance of the Verigene Gram-negative blood culture assay for rapid detection of bacteria and resistance determinants.

Authors:  Magali Dodémont; Ricardo De Mendonça; Claire Nonhoff; Sandrine Roisin; Olivier Denis
Journal:  J Clin Microbiol       Date:  2014-06-04       Impact factor: 5.948
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