BACKGROUND INFORMATION: Commitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing. RESULTS: Here, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effects. Furthermore, semi-quantitative mass spectrometry on purified nuclear mRNPs and chromatin immunoprecipitation analysis on intron-containing genes indicated that H2B ubiquitylation, but not Set1-, Set2- or Dot1-dependent H3 methylation, stimulates recruitment of the early splicing factors, namely U1 and U2 snRNPs, onto nascent RNAs. CONCLUSIONS: These results suggest that histone modifications impact splicing of distinct subsets of genes using distinct pathways.
BACKGROUND INFORMATION: Commitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing. RESULTS: Here, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effects. Furthermore, semi-quantitative mass spectrometry on purified nuclear mRNPs and chromatin immunoprecipitation analysis on intron-containing genes indicated that H2B ubiquitylation, but not Set1-, Set2- or Dot1-dependent H3 methylation, stimulates recruitment of the early splicing factors, namely U1 and U2 snRNPs, onto nascent RNAs. CONCLUSIONS: These results suggest that histone modifications impact splicing of distinct subsets of genes using distinct pathways.
Authors: M S Longtine; A McKenzie; D J Demarini; N G Shah; A Wach; A Brachat; P Philippsen; J R Pringle Journal: Yeast Date: 1998-07 Impact factor: 3.239
Authors: Magdalena Woloszynska; Sabine Le Gall; Pia Neyt; Tommaso M Boccardi; Marion Grasser; Gernot Längst; Stijn Aesaert; Griet Coussens; Stijn Dhondt; Eveline Van De Slijke; Leonardo Bruno; Jorge Fung-Uceda; Paloma Mas; Marc Van Montagu; Dirk Inzé; Kristiina Himanen; Geert De Jaeger; Klaus D Grasser; Mieke Van Lijsebettens Journal: Proc Natl Acad Sci U S A Date: 2019-03-28 Impact factor: 11.205
Authors: Paula Oliete-Calvo; Joan Serrano-Quílez; Carme Nuño-Cabanes; María E Pérez-Martínez; Luis M Soares; Bernhard Dichtl; Stephen Buratowski; José E Pérez-Ortín; Susana Rodríguez-Navarro Journal: EMBO Rep Date: 2018-09-24 Impact factor: 8.807
Authors: Matthew R Sorenson; Deepak K Jha; Stefanie A Ucles; Danielle M Flood; Brian D Strahl; Scott W Stevens; Tracy L Kress Journal: RNA Biol Date: 2016-01-29 Impact factor: 4.652