BACKGROUND: Phosphatidylethanol (PEth) is a direct marker of alcohol consumption, which has been known for almost 30 years. Each PEth molecule carries 2 fatty acids, which differ in chain length and degree of unsaturation. It is formed by means of phospholipase D in the presence of ethanol. Usually, this marker was used by quantification of the PEth homologue 16:0/18:1. The intention of this work was to get more information about the distribution and the quantity of the different PEth homologues. METHODS: Blood samples from 12 alcohol-dependent subjects were collected and analyzed during withdrawal therapy. For comparison, blood from 78 healthy social drinkers was also analyzed. PEth analysis was performed as follows: after liquid-liquid extraction, the homologues were separated on a Luna Phenyl Hexyl column, injected to an HPLC system (1100 system; Agilent) and identified by ESI-MS/MS (QTrap 2000; AB Sciex) using multiple reaction monitoring. RESULTS: PEth 16:0/18:1 is the major homologue comparing the area ratios of PEth homologues in blood samples from alcoholics. Additional prevalent homologues were PEth 16:0/18:2, 18:0/18:2, and 18:0/18:1. The homologues occurring in blood samples from alcoholics as well as from social drinkers were mostly the same, but differences among their distribution pattern were observed. CONCLUSIONS: In addition to the approach to quantitate the PEth homologue 16:0/18:1, this is a new and alternative proceeding for the differentiation between alcoholics and social drinkers using this alcohol consumption marker.
BACKGROUND:Phosphatidylethanol (PEth) is a direct marker of alcohol consumption, which has been known for almost 30 years. Each PEth molecule carries 2 fatty acids, which differ in chain length and degree of unsaturation. It is formed by means of phospholipase D in the presence of ethanol. Usually, this marker was used by quantification of the PEth homologue 16:0/18:1. The intention of this work was to get more information about the distribution and the quantity of the different PEth homologues. METHODS: Blood samples from 12 alcohol-dependent subjects were collected and analyzed during withdrawal therapy. For comparison, blood from 78 healthy social drinkers was also analyzed. PEth analysis was performed as follows: after liquid-liquid extraction, the homologues were separated on a Luna Phenyl Hexyl column, injected to an HPLC system (1100 system; Agilent) and identified by ESI-MS/MS (QTrap 2000; AB Sciex) using multiple reaction monitoring. RESULTS:PEth 16:0/18:1 is the major homologue comparing the area ratios of PEth homologues in blood samples from alcoholics. Additional prevalent homologues were PEth 16:0/18:2, 18:0/18:2, and 18:0/18:1. The homologues occurring in blood samples from alcoholics as well as from social drinkers were mostly the same, but differences among their distribution pattern were observed. CONCLUSIONS: In addition to the approach to quantitate the PEth homologue 16:0/18:1, this is a new and alternative proceeding for the differentiation between alcoholics and social drinkers using this alcohol consumption marker.
Authors: Peter M Thompson; Nathalie Hill-Kapturczak; Marisa Lopez-Cruzan; Luis A Alvarado; Alok K Dwivedi; Martin A Javors Journal: Alcohol Clin Exp Res Date: 2016-11-03 Impact factor: 3.455
Authors: Nathalie Hill-Kapturczak; Donald M Dougherty; John D Roache; Tara E Karns-Wright; Martin A Javors Journal: Alcohol Clin Exp Res Date: 2018-03-30 Impact factor: 3.455
Authors: Martin A Javors; Nathalie Hill-Kapturczak; John D Roache; Tara E Karns-Wright; Donald M Dougherty Journal: Alcohol Clin Exp Res Date: 2016-04-30 Impact factor: 3.455
Authors: Marisa Lopez-Cruzan; John D Roache; Nathalie Hill-Kapturczak; Tara E Karns-Wright; Donald M Dougherty; Jesus J Sanchez; Wouter Koek; Martin A Javors Journal: Alcohol Clin Exp Res Date: 2018-08-26 Impact factor: 3.455
Authors: Judith A Hahn; Pamela M Murnane; Eric Vittinghoff; Winnie R Muyindike; Nneka I Emenyonu; Robin Fatch; Gabriel Chamie; Jessica E Haberer; Joel M Francis; Saidi Kapiga; Karen Jacobson; Bronwyn Myers; Marie Claude Couture; Ralph J DiClemente; Jennifer L Brown; Kaku So-Armah; Mark Sulkowski; Gregory M Marcus; Sarah Woolf-King; Robert L Cook; Veronica L Richards; Patricia Molina; Tekeda Ferguson; David Welsh; Mariann R Piano; Shane A Phillips; Scott Stewart; Majid Afshar; Kimberly Page; Kathleen McGinnis; David A Fiellin; Amy C Justice; Kendall Bryant; Richard Saitz Journal: Alcohol Clin Exp Res Date: 2021-05-07 Impact factor: 3.928