BACKGROUND: The purpose of this study was to characterize the pharmacokinetics of 2 homologues of phosphatidylethanol (PEth) and their combined total in uncoagulated, whole blood samples taken from participants in a human clinical laboratory study after consumption of low doses of ethanol (EtOH). METHODS: As part of a larger study, 14 male and 13 female participants received either 0.25 or 0.50 g/kg oral doses of EtOH during a 15-minute period. Blood samples were collected before and throughout 6 hours after each EtOH dose on the day of consumption and then every 3 days during the next 14 days. PEth 16:0/18:1 and PEth 16:0/18:2 levels were quantified in blood samples by HPLC/MS/MS and reported separately or as their combined total (combined PEth). Breath alcohol concentrations (BrACs) were measured concurrently with each blood collection. Transdermal alcohol concentrations were measured every 30 minutes during the entire 22-day study to confirm the absence of drinking during a 7-day period before and the 14-day period after EtOH consumption. RESULTS: (i) Single doses of 0.25 and 0.50 g EtOH/kg produced proportional increases in BrAC and combined PEth levels of all participants; (ii) the areas under the curve (AUCs) for each participant's BrAC levels during the 6-hour period after EtOH administration were correlated with AUCs of cPEth (calculated as the AUC of the increase above baseline for combined PEth); (iii) the mean half-life of combined PEth, determined during the 14-day period after EtOH consumption, was 4.6 ± 3.5 (SD) days (range: 1.0 to 13.1 days). CONCLUSIONS: Combined PEth is a sensitive biomarker for the identification of relatively low levels of EtOH consumption. The measurement of these 2 homologues may provide additional sensitivity to identify low levels of drinking.
BACKGROUND: The purpose of this study was to characterize the pharmacokinetics of 2 homologues of phosphatidylethanol (PEth) and their combined total in uncoagulated, whole blood samples taken from participants in a human clinical laboratory study after consumption of low doses of ethanol (EtOH). METHODS: As part of a larger study, 14 male and 13 female participants received either 0.25 or 0.50 g/kg oral doses of EtOH during a 15-minute period. Blood samples were collected before and throughout 6 hours after each EtOH dose on the day of consumption and then every 3 days during the next 14 days. PEth 16:0/18:1 and PEth 16:0/18:2 levels were quantified in blood samples by HPLC/MS/MS and reported separately or as their combined total (combined PEth). Breathalcohol concentrations (BrACs) were measured concurrently with each blood collection. Transdermal alcohol concentrations were measured every 30 minutes during the entire 22-day study to confirm the absence of drinking during a 7-day period before and the 14-day period after EtOH consumption. RESULTS: (i) Single doses of 0.25 and 0.50 g EtOH/kg produced proportional increases in BrAC and combined PEth levels of all participants; (ii) the areas under the curve (AUCs) for each participant's BrAC levels during the 6-hour period after EtOH administration were correlated with AUCs of cPEth (calculated as the AUC of the increase above baseline for combined PEth); (iii) the mean half-life of combined PEth, determined during the 14-day period after EtOH consumption, was 4.6 ± 3.5 (SD) days (range: 1.0 to 13.1 days). CONCLUSIONS: Combined PEth is a sensitive biomarker for the identification of relatively low levels of EtOH consumption. The measurement of these 2 homologues may provide additional sensitivity to identify low levels of drinking.
Authors: Paul Marques; Scott Tippetts; John Allen; Martin Javors; Christer Alling; Michel Yegles; Fritz Pragst; Friedrich Wurst Journal: Addiction Date: 2009-11-16 Impact factor: 6.526
Authors: Donald M Dougherty; Nathalie Hill-Kapturczak; Yuanyuan Liang; Tara E Karns; Sarah L Lake; Sharon E Cates; John D Roache Journal: Addict Disord Their Treat Date: 2015-09
Authors: Michael G McDonell; Jordan Skalisky; Emily Leickly; Michael F Orr; Sterling McPherson; John Roll; Nathalie Hill-Kapturczak; Martin Javors Journal: Psychol Addict Behav Date: 2017-07-17
Authors: Andrew K Littlefield; Jennifer L Brown; Ralph J DiClemente; Polina Safonova; Jessica M Sales; Eve S Rose; Nikolay Belyakov; Vadim V Rassokhin Journal: AIDS Behav Date: 2017-07
Authors: Kelly E Moore; Olga J Santiago Rivera; Bradley Anderson; Jennifer E Johnson; Judith A Hahn; Megan E Kurth; Madhavi K Reddy; Yael C Schonbrun; Michael D Stein Journal: Alcohol Clin Exp Res Date: 2018-01-31 Impact factor: 3.455
Authors: Hilke Andresen-Streichert; Alexander Müller; Alexander Glahn; Gisela Skopp; Martina Sterneck Journal: Dtsch Arztebl Int Date: 2018-05-04 Impact factor: 5.594
Authors: Peter M Thompson; Nathalie Hill-Kapturczak; Marisa Lopez-Cruzan; Luis A Alvarado; Alok K Dwivedi; Martin A Javors Journal: Alcohol Clin Exp Res Date: 2016-11-03 Impact factor: 3.455
Authors: Nathalie Hill-Kapturczak; Donald M Dougherty; John D Roache; Tara E Karns-Wright; Martin A Javors Journal: Alcohol Clin Exp Res Date: 2018-03-30 Impact factor: 3.455
Authors: Oghenowede Eyawo; Kathleen A McGinnis; Amy C Justice; David A Fiellin; Judith A Hahn; Emily C Williams; Adam J Gordon; Brandon D L Marshall; Kevin L Kraemer; Stephen Crystal; Julie R Gaither; E Jennifer Edelman; Kendall J Bryant; Janet P Tate Journal: J Acquir Immune Defic Syndr Date: 2018-02-01 Impact factor: 3.731