| Literature DB >> 24434208 |
Lu Wang1, Zibo Zhao1, Mark B Meyer2, Sandeep Saha3, Menggang Yu3, Ailan Guo4, Kari B Wisinski5, Wei Huang6, Weibo Cai7, J Wesley Pike2, Ming Yuan8, Paul Ahlquist9, Wei Xu10.
Abstract
Coactivator-associated arginine methyltransferase 1 (CARM1), a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of BAF155 at R1064 was found to be an independent prognostic biomarker for cancer recurrence and to regulate breast cancer cell migration and metastasis. Furthermore, CARM1-mediated BAF155 methylation affects gene expression by directing methylated BAF155 to unique chromatin regions (e.g., c-Myc pathway genes). Collectively, our studies uncover a mechanism by which BAF155 acquires tumorigenic functions via arginine methylation.Entities:
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Year: 2014 PMID： 24434208 PMCID： PMC4004525 DOI： 10.1016/j.ccr.2013.12.007
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743