| Literature DB >> 2443127 |
A N Roberts, L Barnett, S Brenner.
Abstract
We report the development of a plasmid-mediated transformation system for Arthrobacter sp. NRRLB3381, using the Streptomyces cloning vector pIJ702. Our procedure gives a transformation frequency of 10(3)/micrograms of plasmid DNA. In addition we have explored the expression of the Arthrobacter ermA gene in Streptomyces lividans and Escherichia coli, and shown that the ermA promoter is recognized in S. lividans not E. coli. The relationship between Arthrobacter, Streptomyces and E. coli promoters is discussed.Entities:
Mesh:
Substances:
Year: 1987 PMID: 2443127 PMCID: PMC1147873 DOI: 10.1042/bj2430431
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857