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Literature DB >> 24488339

The isolation of barley-aleurone protoplasts.

Abstract

A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with "Onozuka" cellulase protoplasts were obtained which were surrounded by a thin, "Onozuka"-resistant wall. These cells were termed "spheroplasts". Treatment of spheroplasts with "Glusulase" digested away the residual wall, yielding naked protoplasts. Measurements of respiration using a Clarke-type oxygen electrode indicated that aleurone cells isolated by this procedure were viable.

Entities: Chemical Species

Year: 1971 PMID： 24488339 DOI： 10.1007/BF00387620

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

7 in total

1. A morphological and biochemical analysis of the early phases of cellular growth in the root tip of Vicia faba.

Authors: W A JENSEN
Journal: Exp Cell Res Date: 1955-06 Impact factor: 3.905

2. Gibberellic acid, β-1,3-glucanase and the cell walls of barley aleurone layers.

Authors: L Taiz; R L Jones
Journal: Planta Date: 1970-03 Impact factor: 4.116

3. The fine structure of barley aleurone cells.

Authors: R L Jones
Journal: Planta Date: 1969-12 Impact factor: 4.116

4. Nutrient requirements of suspension cultures of soybean root cells.

Authors: O L Gamborg; R A Miller; K Ojima
Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

5. A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.

Authors: P Filner; J E Varner
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

6. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers.

Authors: M J Chrispeels; J E Varner
Journal: Plant Physiol Date: 1967-03 Impact factor: 8.340

7. Gibberellic Acid-induced synthesis of protease by isolated aleurone layers of barley.

Authors: J V Jacobsen; J E Varner
Journal: Plant Physiol Date: 1967-11 Impact factor: 8.340

7 in total

1. Purification and viability determinations of plant protoplasts.

Authors: P J Larkin
Journal: Planta Date: 1976-01 Impact factor: 4.116

2. Controlled production of spheroplasts in the yeast Nadsonia elongata.

Authors: M Havelková
Journal: Arch Mikrobiol Date: 1973-03-02

3. Experiments on tissue culture in the genus Lycopersicon miller : Mesophyll protoplast regeneration to plants in Lycopersicon esculentum cv. 'Nadja'.

Authors: H Koblitz; D Koblitz
Journal: Plant Cell Rep Date: 1982-06 Impact factor: 4.570

4. The induction of sensitivity to gibberellin in aleurone tissue of developing wheat grains : III. Sensitisation of isolated protoplasts.

Authors: H A Norman; M Black; J M Chapman
Journal: Planta Date: 1983-05 Impact factor: 4.116

5. Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley.

Authors: J V Jacobsen; J A Zwar; P M Chandler
Journal: Planta Date: 1985-03 Impact factor: 4.116

6. Protoplasts isolated from aleurone layers of wild oat (Avena fatua L.) exhibit the classic response to gibberellic acid.

Authors: R Hooley
Journal: Planta Date: 1982-03 Impact factor: 4.116

7. Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen Gene Ara h 2.

Authors: Sudip Biswas; Nancy J Wahl; Michael J Thomson; John M Cason; Bill F McCutchen; Endang M Septiningsih
Journal: Int J Mol Sci Date: 2022-01-13 Impact factor: 5.923

7 in total