C A Avignone Rossa1, O M Yantorno, J A Arcas, R J Ertola. 1. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), Facultad de Ciencias Exactas (Universidad Nacional de La Plata), 47 y 115, 1900, La Plata, Argentina.
Abstract
The influence of different organic and inorganic nitrogen source combinations and C∶N ratios was studied in connection with growth and protein production ofBacillus thuringiensis var.israelensis. Protein production was assumed to be proportional to delta-endotoxin production. Delta-endotoxin concentration increased when media were supplemented with (NH4)2SO4, but the delta-endotoxin: biomass dry weight ratio was unaffected by different C∶N ratios. Organic nitrogen source, yeast extract, could be partially replaced by (NH4)2SO4 with a significant increase in delta-endotoxin production.
The influence of different organic and inn class="Chemical">organicnitrogen source combinations and C∶N ratios was studied in connection with growth and protein production ofBacillus thuringiensis var.israelensis. Protein production was assumed to be proportional to delta-endotoxin production. Delta-endotoxin concentration increased when media were supplemented with (NH4)2SO4, but the delta-endotoxin: biomass dry weight ratio was unaffected by different C∶N ratios. Organicnitrogen source, yeast extract, could be partially replaced by (NH4)2SO4 with a significant increase in delta-endotoxin production.