Literature DB >> 24425261

Coumarin inhibition of microfibril formation at the surface of cultured protoplasts.

J Burgess1, P J Linstead.   

Abstract

The effect of including coumarin in the culture medium of isolated tobacco leaf protoplasts has been studied by scanning microscopy. Fibre formation is completely suppressed for 48 h in concentrations of 200 and 250 mg/l of coumarin. Continuous culture in these concentrations of coumarin does not result in cell division or colony formation, although an atypical wall is visible after 4 days. The effect of coumarin is reversible. Viability of protoplasts exposed for 48 h to 200 mg/l coumarin is unaffected in terms of plating efficiency and infectability with virus. These results are discussed in relation to cell wall synthesis and the possible use of coumarin in prolonging the protoplast state.

Entities:  

Year:  1977        PMID: 24425261     DOI: 10.1007/BF00380688

Source DB:  PubMed          Journal:  Planta        ISSN: 0032-0935            Impact factor:   4.116


  13 in total

1.  Plasmalemma structure in relation to microfibril biosynthesis in Oocystis.

Authors:  D G Robinson; R D Preston
Journal:  Planta       Date:  1972-09       Impact factor: 4.116

2.  Plating of isolated tobacco mesophyll protoplasts on agar medium.

Authors:  T Nagata; I Takebe
Journal:  Planta       Date:  1971-03       Impact factor: 4.116

3.  Ultrastructural studies of the aggregation and fusion of plant protoplasts.

Authors:  J Burgess; E N Fleming
Journal:  Planta       Date:  1974-09       Impact factor: 4.116

Review 4.  The relationship between the plasmalemma and plant cell wall.

Authors:  J C Roland
Journal:  Int Rev Cytol       Date:  1973

5.  Electron microscopic observations of cell regeneration from cultured protoplasts of Ammi visnaga.

Authors:  L C Fowke; C W Bech-Hansen; O L Gamborg
Journal:  Protoplasma       Date:  1974       Impact factor: 3.356

6.  Cellulose microfibril deposition at the plasmalemma surface of regenerating tobacco mesophyll protoplasts: A deep-etch study.

Authors:  B W Grout
Journal:  Planta       Date:  1975-01       Impact factor: 4.116

7.  Ultrastructural studies of the binding of concanavalin A to the plasmalemma of higher plant protoplasts.

Authors:  J Burgess; P J Linstead
Journal:  Planta       Date:  1976-01       Impact factor: 4.116

8.  Cellulose microfibrils: visualization of biosynthetic and orienting complexes in association with the plasma membrane.

Authors:  R M Brown; D Montezinos
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

9.  Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

Authors:  I Takebe; Y Otsuki
Journal:  Proc Natl Acad Sci U S A       Date:  1969-11       Impact factor: 11.205

10.  Ultrastructural observations of cell wall regeneration around isolated tobacco protoplasts.

Authors:  J Burgess; E N Fleming
Journal:  J Cell Sci       Date:  1974-03       Impact factor: 5.285

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  9 in total

1.  The role of microtubules and cell-wall deposition in elongation of regenerating protoplasts of Mougeotia.

Authors:  H J Marchant; E R Hines
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

2.  Structure and association of wall fibrils produced by regenerating tobacco protoplasts.

Authors:  J Burgess; P J Linstead
Journal:  Planta       Date:  1979-01       Impact factor: 4.116

3.  Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division, in protoplasts of Nicotiana tabacum L. cultivated in vitro.

Authors:  Y Meyer; W Herth
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

4.  Cell wall regeneration by protoplasts of Chlamydomonas.

Authors:  D G Robinson; U G Schlösser
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

5.  Observations on the time course of wall development at the surface of isolated protoplasts.

Authors:  J Burgess; P J Linstead; V E Bonsall
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

6.  Further observations on cell-wall formation around isolated protoplasts of tobacco and tomato.

Authors:  J H Willison; B W Grout
Journal:  Planta       Date:  1978-01       Impact factor: 4.116

7.  Characterization of inhibitors of cellulose synthesis in cotton fibers.

Authors:  D Montezinos; D P Delmer
Journal:  Planta       Date:  1980-04       Impact factor: 4.116

8.  Membrane mobility and the Concanavalin A binding system of the plasmalemma of higher plant protoplasts.

Authors:  J Burgess; P J Linstead
Journal:  Planta       Date:  1977-01       Impact factor: 4.116

9.  Pectins as mediators of wall porosity in soybean cells.

Authors:  O Baron-Epel; P K Gharyal; M Schindler
Journal:  Planta       Date:  1988-09       Impact factor: 4.116

  9 in total

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