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Literature DB >> 4905990

Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

Abstract

It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.

Entities: Chemical Disease Species

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Year: 1969 PMID： 4905990 PMCID： PMC223311 DOI： 10.1073/pnas.64.3.843

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

5 in total

1. Relation between incorporation of 2-thiouracil into tobacco mosaic virus nucleic acid and virus inhibition.

Authors: R I FRANCKI; R E MATTHEWS
Journal: Virology Date: 1962-05 Impact factor: 3.616

2. Protoplasts: preparation from higher plants.

Authors: A W Ruesink; K V Thimanndagger
Journal: Science Date: 1966-10-14 Impact factor: 47.728

Review 3. Uptake of protein by mammalian cells: an underdeveloped area. The penetration of foreign proteins into mammalian cells can be measured and their functions explored.

Authors: H J Ryser
Journal: Science Date: 1968-01-26 Impact factor: 47.728

4. Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts.

Authors: Y Otsuki; I Takebe
Journal: Virology Date: 1969-07 Impact factor: 3.616

5. Amino acid sequence of a common Japanese strain of tobacco mosaic virus.

Authors: Y Nozu; Y Okada
Journal: J Mol Biol Date: 1968-08-14 Impact factor: 5.469

5 in total

26 in total

1. The use of protoplasts for genetic research.

Authors: P S Carlson
Journal: Proc Natl Acad Sci U S A Date: 1973-02 Impact factor: 11.205

Review 2. Insights into the single-cell reproduction cycle of members of the family Bromoviridae: lessons from the use of protoplast systems.

Authors: Joanna Sztuba-Solinska; Jozef J Bujarski
Journal: J Virol Date: 2008-08-06 Impact factor: 5.103

7. Conjugation of poly-L-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins.

Authors: W C Shen; H J Ryser
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

1. Relation between incorporation of 2-thiouracil into tobacco mosaic virus nucleic acid and virus inhibition.

2. Protoplasts: preparation from higher plants.

Review 3. Uptake of protein by mammalian cells: an underdeveloped area. The penetration of foreign proteins into mammalian cells can be measured and their functions explored.

4. Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts.

5. Amino acid sequence of a common Japanese strain of tobacco mosaic virus.

1. The use of protoplasts for genetic research.

Review 2. Insights into the single-cell reproduction cycle of members of the family Bromoviridae: lessons from the use of protoplast systems.

3. Structural changes accompanying infection of tobacco protoplasts with two spherical viruses.

4. Surface charge of protoplasts and their significance in cell-cell interaction.

5. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts.

6. Plating of isolated tobacco mesophyll protoplasts on agar medium.

7. Conjugation of poly-L-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins.

8. Photosynthetic activities of spinach leaf protoplasts.

9. Effect of poly-L-ornithine on isolated tobacco mesophyll protoplasts: Evidence against stimulated pinocytosis.

10. The mechanism of infection of plant protoplasts by viruses.