Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Membrane mobility and the Concanavalin A binding system of the plasmalemma of higher plant protoplasts.

Literature DB >> 24420399

Membrane mobility and the Concanavalin A binding system of the plasmalemma of higher plant protoplasts.

Abstract

The binding of a colloidal gold-Concanavalin A (ConA) complex to the plasmalemma of tobacco leaf protoplasts has been investigated using scanning electron microscopy. At 5° C the particles of gold-ConA appear to be randomly distributed over the surface of the protoplast. If the temperature is raised, the particles associate into clusters. Saturation of the membrane with particles can only occur when the weight of ConA in solution exceeds 1 μg/10(4) protoplasts in suspension, and when its concentration exceeds 15 μg/ml. These results are discussed in terms of the properties of the ConA binding site and the mobility of such sites within the membrane surface.

Entities: Chemical Species

Year: 1977 PMID： 24420399 DOI： 10.1007/BF00385993

Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116

17 in total

9. Differences in density of Concanavalin A-binding sites due to differences in surface morphology of suspended normal and transformed 3T3 fibroblasts.

Authors: J G Collard; J H Temmink
Journal: J Cell Sci Date: 1975-10 Impact factor: 5.285

Membrane mobility and the Concanavalin A binding system of the plasmalemma of higher plant protoplasts.

1. Receptor mobility and the binding of cells to lectin-coated fibers.

2. Ultrastructural studies of the aggregation and fusion of plant protoplasts.

3. Temperature effects on the concanavalin A molecule and on concanavalin A binding.

4. Inhibition of lectin agglutinability by fixation of the cell surface membrane.

5. A comparative study of four cytochemical detection methods of concanavalin A binding sites on the cell membrane.

6. Ultrastructural studies of the binding of concanavalin A to the plasmalemma of higher plant protoplasts.

7. Coumarin inhibition of microfibril formation at the surface of cultured protoplasts.

8. The fluid mosaic model of the structure of cell membranes.

9. Differences in density of Concanavalin A-binding sites due to differences in surface morphology of suspended normal and transformed 3T3 fibroblasts.

10. Effect on glutaraldehyde fixation on lectin-mediated agglutination of mouse leukaemia cells.

1. Structure and association of wall fibrils produced by regenerating tobacco protoplasts.

2. An improved procedure for the isolation and purification of protoplasts from carrot suspension culture.

3. Concanavalin A binding sites on the plasma membrane of leek stem protoplasts.