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Literature DB >> 24423268

Labeling of a protein with fluorophores using maleimide derivitization.

Abstract

Maleimide derivitization of a protein is an essential tool for putting probes such as fluorescent labels at different sites within a polypeptide chain. This allows one to better understand protein-protein or protein-nucleic acid interactions using various biophysical approaches such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).

Entities: Chemical

Keywords: Cysteines; Efficiency of labeling calculation; Fluorophores; Maleimide derivitization; Maleimide modification; Protein labeling

Mesh：

Substances：

Year: 2014 PMID： 24423268 DOI： 10.1016/B978-0-12-420070-8.00007-6

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

15 in total

1. Photostable and Proteolysis-Resistant Förster Resonance Energy Transfer-Based Calcium Biosensor.

Authors: Dat Nguyen; Danielle M Behrens; Sanjana Sen; Avid Najdahmadi; Jessica N Pham; Gaetano Speciale; Micah M Lawrence; Sudipta Majumdar; Gregory A Weiss; Elliot L Botvinick
Journal: Anal Chem Date: 2020-05-13 Impact factor: 6.986

2. Cysteine-Targeted Insecticides against A. gambiae Acetylcholinesterase Are Neither Selective nor Reversible Inhibitors.

Authors: Lukas Gorecki; Rudolf Andrys; Monika Schmidt; Tomas Kucera; Miroslav Psotka; Barbora Svobodova; Veronika Hrabcova; Vendula Hepnarova; Petr Bzonek; Daniel Jun; Kamil Kuca; Jan Korabecny; Kamil Musilek
Journal: ACS Med Chem Lett Date: 2019-11-26 Impact factor: 4.345

3. Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB⁺¹.

Authors: Michal Chojnacki; Daoning Zhang; Monika Talarowska; Piotr Gałecki; Janusz Szemraj; David Fushman; Mark A Nakasone
Journal: FEBS Lett Date: 2016-11-22 Impact factor: 4.124

4. Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls.

Authors: Neus Gacias-Amengual; Lena Wohlschlager; Florian Csarman; Roland Ludwig
Journal: Int J Mol Sci Date: 2022-05-06 Impact factor: 6.208

5. 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM).

Authors: Nils Rouven Hansmeier; Ina Sophie Büschlen; Rose Yinghan Behncke; Sascha Ulferts; Radjesh Bisoendial; René Hägerling
Journal: Int J Mol Sci Date: 2022-04-15 Impact factor: 6.208

Labeling of a protein with fluorophores using maleimide derivitization.

1. Photostable and Proteolysis-Resistant Förster Resonance Energy Transfer-Based Calcium Biosensor.

2. Cysteine-Targeted Insecticides against A. gambiae Acetylcholinesterase Are Neither Selective nor Reversible Inhibitors.

3. Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB⁺¹.

4. Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls.

5. 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM).

Review 6. Single-Molecule Fluorescence Applied to Translation.

7. Cell-free reconstitution of multi-condensate assemblies.

8. Rapid Estimation of Membrane Protein Orientation in Liposomes.

9. Site-Specific Labeling of Proteins with Near-IR Heptamethine Cyanine Dyes.

10. Heterodimer Formation of the Homodimeric ABC Transporter OpuA.