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Literature DB >> 24419620

Purification, crystallization and preliminary X-ray analysis of an HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor toxin.

Chikako Iwasa¹, Takashi Tonozuka¹, Masaya Shinoda¹, Yoshimasa Sagane², Koichi Niwa², Toshihiro Watanabe², Hiromi Yoshida³, Shigehiro Kamitori³, Toshifumi Takao⁴, Keiji Oguma⁵, Atsushi Nishikawa¹.

Abstract

The haemagglutinin (HA) complex of Clostridium botulinum type C toxin is composed of three types of subcomponents: HA33, HA17 and HA70 (also known as HA1, HA2 and HA3, respectively). Here, a 260 kDa HA17-HA70 complex was crystallized. His-tagged HA17 and maltose-binding-protein-tagged HA70 were expressed in Escherichia coli and their complex was affinity-purified using a combination of amylose resin chromatography and nickel-nitrilotriacetic acid agarose chromatography. Diffraction data were collected to 8.0 Å resolution and the crystal belonged to the tetragonal space group P4(1)2(1)2. The molecular-replacement solution indicated that one molecule of HA17 was bound to each HA70 monomer.

Entities: Chemical Species

Keywords: Clostridium botulinum; haemagglutinin; lectins; neurotoxins

Mesh：

Substances：
Protein Subunits

Year: 2013 PMID： 24419620 PMCID： PMC3943108 DOI： 10.1107/S2053230X13032378

Source DB: PubMed Journal: Acta Crystallogr F Struct Biol Commun ISSN： 2053-230X Impact factor: 1.056

19 in total

1. In vitro reconstitution of the Clostridium botulinum type D progenitor toxin.

Authors: Hirokazu Kouguchi; Toshihiro Watanabe; Yoshimasa Sagane; Hiroyuki Sunagawa; Tohru Ohyama
Journal: J Biol Chem Date: 2001-11-16 Impact factor: 5.157

2. Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals.

Authors: Katherine A Kantardjieff; Bernhard Rupp
Journal: Protein Sci Date: 2003-09 Impact factor: 6.725

3. Crystal structure of Clostridium botulinum whole hemagglutinin reveals a huge triskelion-shaped molecular complex.

Authors: Sho Amatsu; Yo Sugawara; Takuhiro Matsumura; Kengo Kitadokoro; Yukako Fujinaga
Journal: J Biol Chem Date: 2013-10-28 Impact factor: 5.157

4. Molecular replacement with MOLREP.

Authors: Alexei Vagin; Alexei Teplyakov
Journal: Acta Crystallogr D Biol Crystallogr Date: 2009-12-21

5. Molecular diversity of the two sugar-binding sites of the β-trefoil lectin HA33/C (HA1) from Clostridium botulinum type C neurotoxin.

Authors: Toshio Nakamura; Takashi Tonozuka; Sakae Ito; Yoichi Takeda; Ryutaro Sato; Ichiro Matsuo; Yukishige Ito; Keiji Oguma; Atsushi Nishikawa
Journal: Arch Biochem Biophys Date: 2011-05-26 Impact factor: 4.013

6. Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides.

Authors: Satoshi Yamashita; Hiromi Yoshida; Noboru Uchiyama; Yukari Nakakita; Shin-ichi Nakakita; Takashi Tonozuka; Keiji Oguma; Atsushi Nishikawa; Shigehiro Kamitori
Journal: FEBS Lett Date: 2012-06-07 Impact factor: 4.124

7. Crystal structure of the HA3 subcomponent of Clostridium botulinum type C progenitor toxin.

Authors: Toshio Nakamura; Mao Kotani; Takashi Tonozuka; Azusa Ide; Keiji Oguma; Atsushi Nishikawa
Journal: J Mol Biol Date: 2008-11-27 Impact factor: 5.469

8. Molecular characterization of binding subcomponents of Clostridium botulinum type C progenitor toxin for intestinal epithelial cells and erythrocytes.

Authors: Yukako Fujinaga; Kaoru Inoue; Shinobu Watarai; Yoshihiko Sakaguchi; Hideyuki Arimitsu; Jae-Chul Lee; Yingji Jin; Takuhiro Matsumura; Yuko Kabumoto; Toshihiro Watanabe; Tohru Ohyama; Atsushi Nishikawa; Keiji Oguma
Journal: Microbiology Date: 2004-05 Impact factor: 2.777

Purification, crystallization and preliminary X-ray analysis of an HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor toxin.

1. In vitro reconstitution of the Clostridium botulinum type D progenitor toxin.

2. Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals.

3. Crystal structure of Clostridium botulinum whole hemagglutinin reveals a huge triskelion-shaped molecular complex.

4. Molecular replacement with MOLREP.

5. Molecular diversity of the two sugar-binding sites of the β-trefoil lectin HA33/C (HA1) from Clostridium botulinum type C neurotoxin.

6. Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides.

7. Crystal structure of the HA3 subcomponent of Clostridium botulinum type C progenitor toxin.

8. Molecular characterization of binding subcomponents of Clostridium botulinum type C progenitor toxin for intestinal epithelial cells and erythrocytes.

9. REFMAC5 for the refinement of macromolecular crystal structures.

10. Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.

Review 1. Lectin engineering: the possible and the actual.

2. Glycan detecting tools developed from the Clostridium botulinum whole hemagglutinin complex.