Literature DB >> 24418537

hnRNP A2/B1 interacts with influenza A viral protein NS1 and inhibits virus replication potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nuclear export.

Yimeng Wang1, Jianhong Zhou1, Yuchun Du1.   

Abstract

The NS1 protein of influenza viruses is a major virulence factor and exerts its function through interacting with viral/cellular RNAs and proteins. In this study, we identified heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as an interacting partner of NS1 proteins by a proteomic method. Knockdown of hnRNP A2/B1 by small interfering RNA (siRNA) resulted in higher levels of NS vRNA, NS1 mRNA, and NS1 protein in the virus-infected cells. In addition, we demonstrated that hnRNP A2/B1 proteins are associated with NS1 and NS2 mRNAs and that knockdown of hnRNP A2/B1 promotes transport of NS1 mRNA from the nucleus to the cytoplasm in the infected cells. Lastly, we showed that knockdown of hnRNP A2/B1 leads to enhanced virus replication. Our results suggest that hnRNP A2/B1 plays an inhibitory role in the replication of influenza A virus in host cells potentially through suppressing NS1 RNA/protein levels and NS1 mRNA nucleocytoplasmic translocation.
© 2013 Published by Elsevier Inc.

Entities:  

Keywords:  Influenza A virus; NS1; Protein–protein interaction; Proteomics; hnRNP A2/B1; mRNA nuclear export

Mesh：

Substances：

Year:  2013        PMID： 24418537      PMCID： PMC3911876          DOI： 10.1016/j.virol.2013.11.009

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


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