Literature DB >> 2439438

Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody. III. Demonstration of replication patterns in highly resolved chromosomes.

A Latos-Bielenska, H Hameister, W Vogel.   

Abstract

A bromodeoxyuridine antibody staining technique (BAT) was applied for the analysis of human chromosomes of different chromosomal band resolution. For this purpose lymphocyte cultures were synchronized and labeled with bromodeoxyuridine during the second half of the S-phase. Generally BAT was found comparable to GTG banding though some prominent GTG bands and the constitutive heterochromatin exhibit less intense staining with this technique.

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Year:  1987        PMID： 2439438     DOI： 10.1007/bf00283627

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  12 in total

1.  THE ORGANIZATION AND DUPLICATION OF CHROMOSOMES AS REVEALED BY AUTORADIOGRAPHIC STUDIES USING TRITIUM-LABELED THYMIDINEE.

Authors:  J H Taylor; P S Woods; W L Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  1957-01-15       Impact factor: 11.205

2.  Detection of bromodeoxyuridine incorporation in mammalian chromosomes by a bromodeoxyuridine antibody. II. Demonstration of sister chromatid exchanges.

Authors:  G Speit; W Vogel
Journal:  Chromosoma       Date:  1986       Impact factor: 4.316

3.  Influence of incorporated 5-bromodeoxyuridine on the frequencies of spontaneous and induced sister-chromatid exchanges, detected by immunological methods.

Authors:  A T Natarajan; A H Rotteveel; J van Pieterson; M G Schliermann
Journal:  Mutat Res       Date:  1986-10       Impact factor: 2.433

4.  Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

Authors:  S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes.

Authors:  H G Gratzner; R C Leif; D J Ingram; A Castro
Journal:  Exp Cell Res       Date:  1975-10-01       Impact factor: 3.905

6.  Decrease of DNA synthesis in amniotic fluid cells during the middle part of S-phase revealed by differential chromosome staining after incorporation of BrdU.

Authors:  W Schempp; W Vogel
Journal:  Chromosoma       Date:  1978-06-28       Impact factor: 4.316

7.  Measurement of sister chromatid exchanges at very low bromodeoxyuridine substitution levels using a monoclonal antibody in Chinese hamster ovary cells.

Authors:  D Pinkel; L H Thompson; J W Gray; M Vanderlaan
Journal:  Cancer Res       Date:  1985-11       Impact factor: 12.701

8.  Considerations on the mechanism of differential Giemsa staining of BrdU-substituted chromosomes.

Authors:  G Speit
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

9.  Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns.

Authors:  W Vogel; M Autenrieth; G Speit
Journal:  Hum Genet       Date:  1986-02       Impact factor: 4.132

10.  Asynchronous duplication of chromosomes in cultured cells of Chinese hamster.

Authors:  J H TAYLOR
Journal:  J Biophys Biochem Cytol       Date:  1960-06
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  9 in total

1.  High-resolution dynamic and morphological G-bandings (GBG and GTG): a comparative study.

Authors:  N Lemieux; R Drouin; C L Richer
Journal:  Hum Genet       Date:  1990-08       Impact factor: 4.132

2.  Demonstration of replication patterns in the last premeiotic S-phase of male Chinese hamsters after BrdU pulse labeling.

Authors:  A Latos-Bielenska; W Vogel
Journal:  Chromosoma       Date:  1992-03       Impact factor: 4.316

3.  Dynamic G- and R-banding of human chromosomes for electron microscopy.

Authors:  R Drouin; P E Messier; C L Richer
Journal:  Chromosoma       Date:  1989-06       Impact factor: 4.316

Review 4.  Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part III. Proliferation in normal, injured and diseased tissue, growth factors, differentiation, DNA replication sites and in situ hybridization.

Authors:  F Dolbeare
Journal:  Histochem J       Date:  1996-08

5.  DNA denaturation for ultrastructural banding and the mechanism underlying the fluorochrome-photolysis-Giemsa technique studied with anti-5-bromodeoxyuridine antibodies.

Authors:  R Drouin; P E Messier; C L Richer
Journal:  Chromosoma       Date:  1989-09       Impact factor: 4.316

6.  Frequency and distribution of chiasmata in Syrian hamster spermatocytes studied by the BrdU antibody technique.

Authors:  A Latos-Bielenska; W Vogel
Journal:  Chromosoma       Date:  1990-08       Impact factor: 4.316

7.  Scanning ion analytical microscopy for high-resolution detection of 5-bromo-2'-deoxyuridine incorporation in metaphase chromosomes.

Authors:  C A Bourgeois; R Dennebouy; A Gibaud; M Gerbault-Seureau; B Malfoy; G Slodzian; P Galle; B Dutrillaux
Journal:  Chromosome Res       Date:  1996-12       Impact factor: 5.239

8.  An improved method for chromosome banding after fluorescence in situ hybridization: use of a tetramethylrhodhamine-conjugated BrdU antibody.

Authors:  A De Sario; L De Carli; E Raimondi
Journal:  Cytotechnology       Date:  1991-09       Impact factor: 2.058

9.  Spatial organization of large-scale chromatin domains in the nucleus: a magnified view of single chromosome territories.

Authors:  J Ferreira; G Paolella; C Ramos; A I Lamond
Journal:  J Cell Biol       Date:  1997-12-29       Impact factor: 10.539
  9 in total

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