Literature DB >> 1367701

An improved method for chromosome banding after fluorescence in situ hybridization: use of a tetramethylrhodhamine-conjugated BrdU antibody.

A De Sario1, L De Carli, E Raimondi.   

Abstract

A method for high quality chromosome banding after in situ hybridization with biotinylated probes has been developed. Fluoresceine-conjugated avidin is used for probe detection, while chromosome banding is performed with a tetramethylrodhamine-conjugated anti-BrdU antibody. In this way probe localization and chromosome identification can be performed simultaneously simply by changing the incidental light wavelength.

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Year:  1991        PMID： 1367701     DOI： 10.1007/bf00135639

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  10 in total

1.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

2.  New Giemsa method for the differential staining of sister chromatids.

Authors:  P Perry; S Wolff
Journal:  Nature       Date:  1974-09-13       Impact factor: 49.962

3.  DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.

Authors:  M G Mattei; N Philip; E Passage; J P Moisan; J L Mandel; J F Mattei
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

4.  Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.

Authors:  P Lichter; T Cremer; J Borden; L Manuelidis; D C Ward
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

5.  Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome.

Authors:  J Wolfe; S M Darling; R P Erickson; I W Craig; V J Buckle; P W Rigby; H F Willard; P N Goodfellow
Journal:  J Mol Biol       Date:  1985-04-20       Impact factor: 5.469

6.  Regional mapping of the human placental alkaline phosphatase gene (ALPP) to 2q37 by in situ hybridization.

Authors:  E Raimondi; D Talarico; L Moro; W J Rutter; G Della Valle; L De Carli
Journal:  Cytogenet Cell Genet       Date:  1988

7.  Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns.

Authors:  W Vogel; M Autenrieth; G Speit
Journal:  Hum Genet       Date:  1986-02       Impact factor: 4.132

8.  Translocation of c-abl to "masked" Ph in chronic myeloid leukemia.

Authors:  A Hagemeijer; A de Klein; E Gödde-Salz; C Turc-Carel; E M Smit; A J van Agthoven; G C Grosveld
Journal:  Cancer Genet Cytogenet       Date:  1985-10

9.  Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line.

Authors:  J B Lawrence; C A Villnave; R H Singer
Journal:  Cell       Date:  1988-01-15       Impact factor: 41.582

10.  Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody. III. Demonstration of replication patterns in highly resolved chromosomes.

Authors:  A Latos-Bielenska; H Hameister; W Vogel
Journal:  Hum Genet       Date:  1987-07       Impact factor: 4.132
  10 in total
  1 in total

1.  Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells.

Authors:  Daniela Moralli; Zoia L Monaco
Journal:  PLoS One       Date:  2009-02-16       Impact factor: 3.240
  1 in total

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