| Literature DB >> 24389662 |
An Xiao1, Zhenchao Cheng1, Lei Kong1, Zuoyan Zhu1, Shuo Lin1, Ge Gao1, Bo Zhang1.
Abstract
The CRISPR/Cas or Cas9/guide RNA system is a newly developed, easily engineered and highly effective tool for gene targeting; it has considerable off-target effects in cultured human cells and in several organisms. However, the Cas9/guide RNA target site is too short for existing alignment tools to exhaustively and effectively identify potential off-target sites. CasOT is a local tool designed to find potential off-target sites in any given genome or user-provided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches allowed in the seed and non-seed regions. AVAILABILITY: http://eendb.zfgenetics.org/casot/ CONTACT: zfgenetics@gmail.com or bzhang@pku.edu.cn Supplementary Information: Supplementary data are available at Bioinformatics online.Entities:
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Year: 2014 PMID： 24389662 DOI： 10.1093/bioinformatics/btt764
Source DB: PubMed Journal: Bioinformatics ISSN： 1367-4803 Impact factor: 6.937