Literature DB >> 2438685

Profile of the continuous antigenic regions on the extracellular part of the alpha chain of an acetylcholine receptor.

B Mulac-Jericević, J Kurisaki, M Z Atassi.   

Abstract

Reaction of overlapping synthetic peptides spanning the extracellular part (residues 1-210) of the alpha chain of the Torpedo californica acetylcholine receptor (an alpha 2 beta gamma delta pentamer) with anti-receptor antibodies produced the profiles of the continuous antigenic regions of the correlate segment. Essentially similar profiles were recognized by rabbit and outbred mouse antibodies against isolated receptor or mouse antibodies against membrane-bound receptor. The antigenic sites reside within eight continuous regions: residues 1-14, 25-36, 41-53, 63-75, 102-114, 128-138, 172-182, and 188-198. Five of these regions (the second and the fifth through the eighth) appeared to be immunodominant. Significantly, two of these antigenic regions (i.e., those residing within residues 128-138 and 188-198) coincided with known toxin-binding regions. The antigenic profile suggests that recognition is directed to the intact molecule and only very slightly to the processed (fragmented) protein.

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Year:  1987        PMID： 2438685      PMCID： PMC304929          DOI： 10.1073/pnas.84.11.3633

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  50 in total

1.  Antigenic structure of human haemoglobin. Localization of the antigenic sites of the beta-chain in three host species by synthetic overlapping peptides representing the entire chain.

Authors:  N Yoshioka; M Z Atassi
Journal:  Biochem J       Date:  1986-03-01       Impact factor: 3.857

2.  Antibody recognition of ragweed allergen Ra3: localization of the full profile of the continuous antigenic sites by synthetic overlapping peptides representing the entire protein chain.

Authors:  H Atassi; M Z Atassi
Journal:  Eur J Immunol       Date:  1986-03       Impact factor: 5.532

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.

Authors:  S S Twining; H Lehmann; M Z Atassi
Journal:  Biochem J       Date:  1980-12-01       Impact factor: 3.857

5.  Primary structure of alpha-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence.

Authors:  M Noda; H Takahashi; T Tanabe; M Toyosato; Y Furutani; T Hirose; M Asai; S Inayama; T Miyata; S Numa
Journal:  Nature       Date:  1982-10-28       Impact factor: 49.962

6.  Properties of nicotinic acetylcholine receptors isolated by affinity chromatography on monoclonal antibodies.

Authors:  V A Lennon; M Thompson; J Chen
Journal:  J Biol Chem       Date:  1980-05-25       Impact factor: 5.157

7.  Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.

Authors:  S J Tzartos; D E Rand; B L Einarson; J M Lindstrom
Journal:  J Biol Chem       Date:  1981-08-25       Impact factor: 5.157

8.  Preparation of T-lymphocyte lines and clones with specificities to preselected protein sites by in vitro passage with free synthetic peptides: demonstration with myoglobin sites.

Authors:  M Yoshioka; G S Bixler; M Z Atassi
Journal:  Mol Immunol       Date:  1983-10       Impact factor: 4.407

9.  Region of peptide 125-147 of acetylcholine receptor alpha subunit is exposed at neuromuscular junction and induces experimental autoimmune myasthenia gravis, T-cell immunity, and modulating autoantibodies.

Authors:  V A Lennon; D J McCormick; E H Lambert; G E Griesmann; M Z Atassi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

10.  High affinity binding of alpha-bungarotoxin to the purified alpha-subunit and to its 27,000-dalton proteolytic peptide from Torpedo marmorata acetylcholine receptor. Requirement for sodium dodecyl sulfate.

Authors:  S J Tzartos; J P Changeux
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  10 in total

Review 1.  Ligand-gated ion channels. Homology and diversity.

Authors:  V B Cockcroft; D J Osguthorpe; E A Barnard; A E Friday; G G Lunt
Journal:  Mol Neurobiol       Date:  1990 Fall-Winter       Impact factor: 5.590

Review 2.  The main immunogenic region (MIR) of the nicotinic acetylcholine receptor and the anti-MIR antibodies.

Authors:  S J Tzartos; M T Cung; P Demange; H Loutrari; A Mamalaki; M Marraud; I Papadouli; C Sakarellos; V Tsikaris
Journal:  Mol Neurobiol       Date:  1991       Impact factor: 5.590

3.  Profile of the alpha-bungarotoxin-binding regions on the extracellular part of the alpha-chain of Torpedo californica acetylcholine receptor.

Authors:  B Mulac-Jericevic; M Z Atassi
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857

4.  Mapping by synthetic peptides of the binding sites for acetylcholine receptor on alpha-bungarotoxin.

Authors:  M Z Atassi; C S McDaniel; T Manshouri
Journal:  J Protein Chem       Date:  1988-10

5.  Seventh International Conference on Methods in Protein Sequence Analysis. July 3-8, 1988, West Berlin, F.R.G. Short communications.

Authors: 
Journal:  J Protein Chem       Date:  1988-06

6.  The regions of alpha-neurotoxin binding on the extracellular part of the alpha-subunit of human acetylcholine receptor.

Authors:  B Mulac-Jericevic; T Manshouri; T Yokoi; M Z Atassi
Journal:  J Protein Chem       Date:  1988-04

7.  Analysis of exposed regions on the main extracellular domain of mouse acetylcholine receptor alpha subunit in live muscle cells by binding profiles of antipeptide antibodies.

Authors:  K Jinnai; T Ashizawa; M Z Atassi
Journal:  J Protein Chem       Date:  1994-11

8.  Mapping the extracellular topography of the alpha-chain in free and in membrane-bound acetylcholine receptor by antibodies against overlapping peptides spanning the entire extracellular parts of the chain.

Authors:  M Z Atassi; B Mulac-Jericevic
Journal:  J Protein Chem       Date:  1994-01

9.  The short-neurotoxin-binding regions on the alpha-chain of human and Torpedo californica acetylcholine receptors.

Authors:  K H Ruan; B G Stiles; M Z Atassi
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

10.  Epitope-specific suppression of antibody response in experimental autoimmune myasthenia gravis by a monomethoxypolyethylene glycol conjugate of a myasthenogenic synthetic peptide.

Authors:  M Z Atassi; K H Ruan; K Jinnai; M Oshima; T Ashizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-01       Impact factor: 11.205
  10 in total

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