Federico Abascal1, Iker Irisarri2, Rafael Zardoya3. 1. Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO), Melchor Fernández Almagro, 3, 28029 Madrid, Spain. 2. Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales-CSIC (MNCN-CSIC), José Gutiérrez Abascal 2, 28006 Madrid, Spain. 3. Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales-CSIC (MNCN-CSIC), José Gutiérrez Abascal 2, 28006 Madrid, Spain. Electronic address: rafaz@mncn.csic.es.
Abstract
BACKGROUND: Membrane intrinsic proteins (MIPs) are the proteins in charge of regulating water transport into cells. Because of this essential function, the MIP family is ancient, widespread, and highly diverse. SCOPE OF REVIEW: The rapidly accumulating genomic and transcriptomic data from previously poorly known groups such as unicellular eukaryotes, fungi, green algae, mosses, and non-vertebrate animals are contributing to expand our view of MIP evolution throughout the diversity of life. Here, by analyzing more than 1700 sequences, we provide an updated and comprehensive phylogeny of MIPs MAJOR CONCLUSIONS: The reconstructed phylogeny supports (i) deep orthology of X intrinsic proteins (XIPs; present from unicellular eukaryotes to plants); (ii) that the origin of small intrinsic proteins (SIPs) traces back to the common ancestor of all plants; and (iii) the expansion of aquaglyceroporins (GLPs) in Oomycetes, as well as their loss in vascular plants and in the ancestor of endopterygote insects. Additionally, conserved positions in the protein, and residues involved in glycerol selectivity are reviewed within a phylogenetic framework. Furthermore, functional diversification of human and Arabidopsis paralogs are analyzed in an evolutionary genomic context. GENERAL SIGNIFICANCE: Our results show that while bacteria and archaea generally function with one copy of each a water channel (aquaporin or AQP) and a GLP, recurrent independent expansions have greatly diversified the structures and functions of the different members of both MIP paralog subfamilies throughout eukaryote evolution (and not only in flowering plants and vertebrates, as previously thought). This article is part of a Special Issue entitled Aquaporins.
BACKGROUND: Membrane intrinsic proteins (MIPs) are the proteins in charge of regulating water transport into cells. Because of this essential function, the MIP family is ancient, widespread, and highly diverse. SCOPE OF REVIEW: The rapidly accumulating genomic and transcriptomic data from previously poorly known groups such as unicellular eukaryotes, fungi, green algae, mosses, and non-vertebrate animals are contributing to expand our view of MIP evolution throughout the diversity of life. Here, by analyzing more than 1700 sequences, we provide an updated and comprehensive phylogeny of MIPs MAJOR CONCLUSIONS: The reconstructed phylogeny supports (i) deep orthology of X intrinsic proteins (XIPs; present from unicellular eukaryotes to plants); (ii) that the origin of small intrinsic proteins (SIPs) traces back to the common ancestor of all plants; and (iii) the expansion of aquaglyceroporins (GLPs) in Oomycetes, as well as their loss in vascular plants and in the ancestor of endopterygote insects. Additionally, conserved positions in the protein, and residues involved in glycerol selectivity are reviewed within a phylogenetic framework. Furthermore, functional diversification of human and Arabidopsis paralogs are analyzed in an evolutionary genomic context. GENERAL SIGNIFICANCE: Our results show that while bacteria and archaea generally function with one copy of each a water channel (aquaporin or AQP) and a GLP, recurrent independent expansions have greatly diversified the structures and functions of the different members of both MIP paralog subfamilies throughout eukaryote evolution (and not only in flowering plants and vertebrates, as previously thought). This article is part of a Special Issue entitled Aquaporins.
Authors: Pratyush Routray; Tian Li; Arisa Yamasaki; Akira Yoshinari; Junpei Takano; Won Gyu Choi; Carl E Sams; Daniel M Roberts Journal: Plant Physiol Date: 2018-09-28 Impact factor: 8.340
Authors: Elizabeth Trembath-Reichert; Jonathan Paul Wilson; Shawn E McGlynn; Woodward W Fischer Journal: Proc Natl Acad Sci U S A Date: 2015-03-30 Impact factor: 11.205