Daniele G Fuhrich1, Bruce A Lessey2, Ricardo F Savaris1. 1. Department of Gynecology and Obstetrics, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 2. Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of South Carolina School of Medicine-Greenville, Greenville, South Carolina, USA.
Abstract
OBJECTIVE: To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers. STUDY DESIGN: Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE. RESULTS: The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%. CONCLUSION: The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.
OBJECTIVE: To compare intraobserver and interobserver variation between traditional histological score (HSCORE) and digital HSCORE (D-HSCORE) performed by expert and naive researchers. STUDY DESIGN: Immunohistochemical analysis of beta3 integrin subunit of 100 endometrial biopsies obtained from the midluteal phase of the menstrual cycle were reanalyzed using ImageJ software (D-HSCORE). Mean intensity of 3,3'-diaminobenzidine on endometrial glands was read by an expert (HSCORE) versus inexperienced observer using HSCORE and D-HSCORE. RESULTS: The mean correlation [r(s)(95% CI)] between both methods was 0.86 (0.79-0.90) and highly significant (p < 0.0001) for the experienced individual. The naive researcher overestimated immunostaining, resulting (HSCORE) in negative samples. No discrepancies were seen with D-HSCORE. Interobserver variation for the inexperienced reader was 50% using HSCORE (cutoff 0.7) but 0% with D-HSCORE. Intraobserver variation using ImageJ was 0%. CONCLUSION: The D-HSCORE performed by an inexperienced researcher has high correlation to traditional HSCORE performed by an expert.
Authors: Paul B Miller; Brent A Parnell; Greta Bushnell; Nicholas Tallman; David A Forstein; H Lee Higdon; Jo Kitawaki; Bruce A Lessey Journal: Hum Reprod Date: 2012-01-13 Impact factor: 6.918
Authors: K B Apparao; M J Murray; M A Fritz; W R Meyer; A F Chambers; P R Truong; B A Lessey Journal: J Clin Endocrinol Metab Date: 2001-10 Impact factor: 5.958
Authors: B A Lessey; A J Castelbaum; S W Sawin; C A Buck; R Schinnar; W Bilker; B L Strom Journal: J Clin Endocrinol Metab Date: 1994-08 Impact factor: 5.958
Authors: N R Joshi; R W Su; G V R Chandramouli; S K Khoo; J W Jeong; S L Young; B A Lessey; A T Fazleabas Journal: Hum Reprod Date: 2015-09-14 Impact factor: 6.918
Authors: Ren-Wei Su; Michael R Strug; Niraj R Joshi; Jae-Wook Jeong; Lucio Miele; Bruce A Lessey; Steve L Young; Asgerally T Fazleabas Journal: J Clin Endocrinol Metab Date: 2014-12-29 Impact factor: 5.958
Authors: Michael R Strug; Renwei Su; James E Young; William G Dodds; Valerie I Shavell; Patricia Díaz-Gimeno; Maria Ruíz-Alonso; Carlos Simón; Bruce A Lessey; Richard E Leach; Asgerally T Fazleabas Journal: Hum Reprod Date: 2016-04-26 Impact factor: 6.918
Authors: Michael R Strug; Ren-Wei Su; Tae Hoon Kim; Alessandro Mauriello; Carlo Ticconi; Bruce A Lessey; Steven L Young; Jeong Mook Lim; Jae-Wook Jeong; Asgerally T Fazleabas Journal: FASEB J Date: 2018-01-08 Impact factor: 5.191
Authors: Elise L Ma; Allen D Smith; Neemesh Desai; Lumei Cheung; Marie Hanscom; Bogdan A Stoica; David J Loane; Terez Shea-Donohue; Alan I Faden Journal: Brain Behav Immun Date: 2017-07-01 Impact factor: 7.217