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Literature DB >> 24311246

Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin.

Abstract

Bovine serum albumin has been covalently labeled with Remazol brilliant blue R to provide a substrate for a convenient spectrophotometric assay for protein precipitants. The blue protein is especially useful for measuring protein precipitation by vegetable tannins because its absorption maximum is at a wavelength where plant pigments exhibit minimum absorption. Blue BSA has been used to determine, by competition experiments, the relative affinity of various proteins for tannins. A procedure for purifying condensed tannin from commercially available quebracho extract is described.

Entities: Chemical Gene Species

Year: 1985 PMID： 24311246 DOI： 10.1007/BF01012199

Source DB: PubMed Journal: J Chem Ecol ISSN： 0098-0331 Impact factor: 2.626

14 in total

1. Condensed tannins in grain sorghum: isolation, fractionation, and characterization.

Authors: D H Strumeyer; M J Malin
Journal: J Agric Food Chem Date: 1975 Sep-Oct Impact factor: 5.279

2. Comparison of tannin levels in developing fruit buds of two orchard pear varieties using two techniques, Folin-Denis and protein precipitation assays.

Authors: M F Wilson
Journal: J Chem Ecol Date: 1984-03 Impact factor: 2.626

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

4. Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts.

Authors: J S Martin; M M Martin
Journal: J Chem Ecol Date: 1983-02 Impact factor: 2.626

5. A prestaining method for the quantitative assay of proteins on polyacrylamide gels.

Authors: A Datyner; E D Finnimore
Journal: Anal Biochem Date: 1973-10 Impact factor: 3.365

6. A new ultrasensitive method for the determination of proteolytic activity.

Authors: H Rinderknecht; M C Geokas; P Silverman; B J Haverback
Journal: Clin Chim Acta Date: 1968-08 Impact factor: 3.786

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

8. Condensed tannin purification and characterization of tannin-associated proteins.

Authors: A E Hagerman; L G Butler
Journal: J Agric Food Chem Date: 1980 Sep-Oct Impact factor: 5.279

9. Induction of protein and glycoprotein synthesis in rat submandibular glands by isoproterenol.

Authors: H Mehansho; D M Carlson
Journal: J Biol Chem Date: 1983-05-25 Impact factor: 5.157

10. The specificity of proanthocyanidin-protein interactions.

Authors: A E Hagerman; L G Butler
Journal: J Biol Chem Date: 1981-05-10 Impact factor: 5.157

27 in total

Review 1. Salivary proteins as a defense against dietary tannins.

Authors: Takuya Shimada
Journal: J Chem Ecol Date: 2006-05-23 Impact factor: 2.626

2. Correlations between leaf toughness and phenolics among species in contrasting environments of Australia and New Caledonia.

Authors: Jennifer Read; Gordon D Sanson; Elizabeth Caldwell; Fiona J Clissold; Alex Chatain; Paula Peeters; Byron B Lamont; Michel De Garine-Wichatitsky; Tanguy Jaffré; Stuart Kerr
Journal: Ann Bot Date: 2008-12-19 Impact factor: 4.357

3. Antinutritional effects and ecological significance of dietary condensed tannins may not be due to binding and inhibiting digestive enzymes.

Authors: H J Blytt; T K Guscar; L G Butler
Journal: J Chem Ecol Date: 1988-06 Impact factor: 2.626

Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin.

1. Condensed tannins in grain sorghum: isolation, fractionation, and characterization.

2. Comparison of tannin levels in developing fruit buds of two orchard pear varieties using two techniques, Folin-Denis and protein precipitation assays.

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

4. Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts.

5. A prestaining method for the quantitative assay of proteins on polyacrylamide gels.

6. A new ultrasensitive method for the determination of proteolytic activity.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. Condensed tannin purification and characterization of tannin-associated proteins.

9. Induction of protein and glycoprotein synthesis in rat submandibular glands by isoproterenol.

10. The specificity of proanthocyanidin-protein interactions.

Review 1. Salivary proteins as a defense against dietary tannins.

2. Correlations between leaf toughness and phenolics among species in contrasting environments of Australia and New Caledonia.

3. Antinutritional effects and ecological significance of dietary condensed tannins may not be due to binding and inhibiting digestive enzymes.

4. Choosing appropriate methods and standards for assaying tannin.

5. Extraction of tannin from fresh and preserved leaves.

6. Radial diffusion method for determining tannin in plant extracts.

7. Implications of soluble tannin-protein complexes for tannin analysis and plant defense mechanisms.

8. Extraction of condensed tannins from cervid feed and feces and quantification using a radial diffusion assay.

9. Changes in western wheatgrass foliage quality following defoliation: consequences for a graminivorous grasshopper.

10. Ecological tannin assays: a critique.