| Literature DB >> 24310435 |
N Overbeeke1, M A Haring, H John, J Nijkamp, A J Kool.
Abstract
Sequences from Petunia hybrida chloroplast DNA which have the property to promote autonomous replication in Saccharomyces cerevisiae were cloned in vector YIp5. Seven cloned chloroplast DNA fragments are localized at one of two different sites on the chloroplast genome. One site, arsA was mapped on a 1.8 Kb fragment at position 27.0-28.8 Kb on the P. hybrida chloroplast genome. The plasmids containing this arsA are stable both in yeast and E. coli. The other site, arsB, was shown to be very unstable and is located either in the small single copy region close to the inverted repeat or just in the inverted repeat. The functioning of these sequences as a possible origin of replication in vivo is discussed.Entities:
Year: 1984 PMID: 24310435 DOI: 10.1007/BF00029659
Source DB: PubMed Journal: Plant Mol Biol ISSN: 0167-4412 Impact factor: 4.076