Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Isolation and subcloning analysis of functional centromere DNA (CEN11) from Saccharomyces cerevisiae chromosome XI.

Literature DB >> 6287222

Isolation and subcloning analysis of functional centromere DNA (CEN11) from Saccharomyces cerevisiae chromosome XI.

M Fitzgerald-Hayes, J M Buhler, T G Cooper, J Carbon.

Abstract

We have cloned segments of yeast DNA containing the centromere XI-linked MET14 gene. This was done by selecting directly in Saccharomyces cerevisiae for complementation of a met14 mutation after transformation with a hybrid plasmid DNA genomic library. Genetic evidence indicates that functional centromere DNA (CEN11) from chromosome XI is also contained on the segment of S. cerevisiae DNA cloned in pYe(MET14)2. This plasmid is maintained stably in budding S. cerevisiae cultures and segregates predominantly 2+:20- through meiosis. The CEN11 element has been subcloned in vector YRp7' on an S. cerevisiae DNA fragment 900 base pairs in length [pYe(CEN11)10]. The mitotic and meiotic behavior of plasmids containing CEN11 plus a DNA replicator (ars) indicates that the centromere DNA sequences enable these plasmids to function as true minichromosomes in S. cerevisiae.

Entities: Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6287222 PMCID： PMC369755 DOI： 10.1128/mcb.2.1.82-87.1982

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

18 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Genetic mapping in Saccharomyces.

Authors: R K Mortimer; D C Hawthorne
Journal: Genetics Date: 1966-01 Impact factor: 4.562

3. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

Authors: K A Nasmyth; S I Reed
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

4. Evidence for transposition of dispersed repetitive DNA families in yeast.

Authors: J R Cameron; E Y Loh; R W Davis
Journal: Cell Date: 1979-04 Impact factor: 41.582

5. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.

Authors: A C Chinault; J Carbon
Journal: Gene Date: 1979-02 Impact factor: 3.688

6. Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.

Authors: J McGrew; B Diehl; M Fitzgerald-Hayes
Journal: Mol Cell Biol Date: 1986-02 Impact factor: 4.272

Isolation and subcloning analysis of functional centromere DNA (CEN11) from Saccharomyces cerevisiae chromosome XI.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Genetic mapping in Saccharomyces.

3. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

4. Evidence for transposition of dispersed repetitive DNA families in yeast.

5. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.

6. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

7. Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway.

8. High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.

9. Direct selection procedure for the isolation of functional centromeric DNA.

10. Isolation of a yeast centromere and construction of functional small circular chromosomes.

Review 1. Complex regulation of sister kinetochore orientation in meiosis-I.

2. Mutations in CEN3 cause aberrant chromosome segregation during meiosis in Saccharomyces cerevisiae.

3. Characterization of a centromere-linked recombination hot spot in Saccharomyces cerevisiae.

4. Mitotic recombination among subtelomeric Y' repeats in Saccharomyces cerevisiae.

Review 5. Yeast chromosome replication and segregation.

6. Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.

7. Toxic effects of excess cloned centromeres.

8. Tightly centromere-linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae.

Review 9. Emerging roles for centromeres in meiosis I chromosome segregation.

10. Genetic manipulation of centromere function.