Literature DB >> 24310230

Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F).

Toshiki Ishikawa1, Hiroyuki Imai, Kawai-Yamada Maki.   

Abstract

The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of reliable detection of several fmol of the derivatives. Lipid extracts from plant samples were derivatized with NBD-F, and all nine species of free sphingoid bases present in plant sphingolipids were separated and quantified for the first time; a complete baseline resolution was achieved for cis-8 and trans-8 isomers of sphingoid bases by reversed phase HPLC on a C18 column. The extraction and derivatization procedures and LC-MS/MS method can facilitate the progress of the studies for seeking the active components of sphingoid bases species in response to biological challenges.

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Year:  2013        PMID： 24310230     DOI： 10.1007/s11745-013-3871-6

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  19 in total

1.  An improved method for analysis of glucosylceramide species having cis-8 and trans-8 isomers of sphingoid bases by LC-MS/MS.

Authors:  Hiroyuki Imai; Hideyasu Hattori; Masayuki Watanabe
Journal:  Lipids       Date:  2012-10-30       Impact factor: 1.880

2.  Separation and identification of major plant sphingolipid classes from leaves.

Authors:  Jonathan E Markham; Jia Li; Edgar B Cahoon; Jan G Jaworski
Journal:  J Biol Chem       Date:  2006-06-12       Impact factor: 5.157

3.  Enantioselective two-dimensional high-performance liquid chromatographic determination of N-methyl-D-aspartic acid and its analogues in mammals and bivalves.

Authors:  Reiko Koga; Yurika Miyoshi; Eiichi Negishi; Tsuneaki Kaneko; Masashi Mita; Wolfgang Lindner; Kenji Hamase
Journal:  J Chromatogr A       Date:  2012-08-31       Impact factor: 4.759

4.  Sphingolipid Δ8 unsaturation is important for glucosylceramide biosynthesis and low-temperature performance in Arabidopsis.

Authors:  Ming Chen; Jonathan E Markham; Edgar B Cahoon
Journal:  Plant J       Date:  2011-12-01       Impact factor: 6.417

5.  High-performance liquid chromatography analysis of molecular species of sphingolipid-related long chain bases and long chain base phosphates in Saccharomyces cerevisiae after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate.

Authors:  R L Lester; R C Dickson
Journal:  Anal Biochem       Date:  2001-11-15       Impact factor: 3.365

6.  Fumonisin- and AAL-Toxin-Induced Disruption of Sphingolipid Metabolism with Accumulation of Free Sphingoid Bases.

Authors:  H. K. Abbas; T. Tanaka; S. O. Duke; J. K. Porter; E. M. Wray; L. Hodges; A. E. Sessions; E. Wang; A. H. Merrill; R. T. Riley
Journal:  Plant Physiol       Date:  1994-11       Impact factor: 8.340

7.  Genome-wide insertional mutagenesis of Arabidopsis thaliana.

Authors:  José M Alonso; Anna N Stepanova; Thomas J Leisse; Christopher J Kim; Huaming Chen; Paul Shinn; Denise K Stevenson; Justin Zimmerman; Pascual Barajas; Rosa Cheuk; Carmelita Gadrinab; Collen Heller; Albert Jeske; Eric Koesema; Cristina C Meyers; Holly Parker; Lance Prednis; Yasser Ansari; Nathan Choy; Hashim Deen; Michael Geralt; Nisha Hazari; Emily Hom; Meagan Karnes; Celene Mulholland; Ral Ndubaku; Ian Schmidt; Plinio Guzman; Laura Aguilar-Henonin; Markus Schmid; Detlef Weigel; David E Carter; Trudy Marchand; Eddy Risseeuw; Debra Brogden; Albana Zeko; William L Crosby; Charles C Berry; Joseph R Ecker
Journal:  Science       Date:  2003-08-01       Impact factor: 47.728

8.  Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

Authors:  Jonathan E Markham; Jan G Jaworski
Journal:  Rapid Commun Mass Spectrom       Date:  2007       Impact factor: 2.419

9.  Fatty acid desaturases from the microalga Thalassiosira pseudonana.

Authors:  Thierry Tonon; Olga Sayanova; Louise V Michaelson; Renwei Qing; David Harvey; Tony R Larson; Yi Li; Johnathan A Napier; Ian A Graham
Journal:  FEBS J       Date:  2005-07       Impact factor: 5.542

10.  Functional characterization of a higher plant sphingolipid Delta4-desaturase: defining the role of sphingosine and sphingosine-1-phosphate in Arabidopsis.

Authors:  Louise V Michaelson; Simone Zäuner; Jonathan E Markham; Richard P Haslam; Radhika Desikan; Sarah Mugford; Sandra Albrecht; Dirk Warnecke; Petra Sperling; E Heinz; Johnathan A Napier
Journal:  Plant Physiol       Date:  2008-10-31       Impact factor: 8.340
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  3 in total

1.  Synthesis and degradation of long-chain base phosphates affect fumonisin B1-induced cell death in Arabidopsis thaliana.

Authors:  Daiki Yanagawa; Toshiki Ishikawa; Hiroyuki Imai
Journal:  J Plant Res       Date:  2017-03-16       Impact factor: 2.629

2.  The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato.

Authors:  Ying Zhou; Lanting Zeng; Xiumin Fu; Xin Mei; Sihua Cheng; Yinyin Liao; Rufang Deng; Xinlan Xu; Yueming Jiang; Xuewu Duan; Susanne Baldermann; Ziyin Yang
Journal:  Sci Rep       Date:  2016-12-08       Impact factor: 4.379

3.  Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity.

Authors:  Masaya Sato; Minoru Nagano; Song Jin; Atsuko Miyagi; Masatoshi Yamaguchi; Maki Kawai-Yamada; Toshiki Ishikawa
Journal:  Plants (Basel)       Date:  2019-12-23
  3 in total

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