| Literature DB >> 12893945 |
José M Alonso1, Anna N Stepanova, Thomas J Leisse, Christopher J Kim, Huaming Chen, Paul Shinn, Denise K Stevenson, Justin Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina C Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, Michael Geralt, Nisha Hazari, Emily Hom, Meagan Karnes, Celene Mulholland, Ral Ndubaku, Ian Schmidt, Plinio Guzman, Laura Aguilar-Henonin, Markus Schmid, Detlef Weigel, David E Carter, Trudy Marchand, Eddy Risseeuw, Debra Brogden, Albana Zeko, William L Crosby, Charles C Berry, Joseph R Ecker.
Abstract
Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.Entities:
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Year: 2003 PMID： 12893945 DOI： 10.1126/science.1086391
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728