| Literature DB >> 24302523 |
Abstract
A gene encoding the initiator methionine transfer RNA (tRNA(met-i)) has been isolated from a soybean genomic library. The nucleotide sequence of the gene is colinear with the previously determined sequence of bean and wheat germ tRNA(met-i) (3, 8) except for a transversion at site 51/63 in stem IV. Microinjection of the soybean gene into Xenopus oocytes caused the transcription of an RNA product equal in size to mature Xenopus tRNA(met-i). Hybridization of a restriction fragment containing the tRNA(met-i) gene to digests of soybean DNA reveals that the gene occurs in only one to a few copies per haploid genome.Entities:
Year: 1987 PMID: 24302523 DOI: 10.1007/BF00016433
Source DB: PubMed Journal: Plant Mol Biol ISSN: 0167-4412 Impact factor: 4.076