Literature DB >> 534484

An improved method for the rapid isolation of brain ribonucleic acid.

B B Kaplan, S L Bernstein, A E Gioio.   

Abstract

An improved one-step method for the extraction of RNA from rat brain is described. Fresh or frozen tissue is disrupted in the powerful protein denaturant guanidine thiocyanate, and RNA isolated by ultracentrifugation through CsCl. The procedure is advantageous in that it is relatively simple, is rapid and does not expose the sample to enzyme treatments or repeated organic extractions.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 534484      PMCID： PMC1161490          DOI： 10.1042/bj1830181

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  Structural gene sets active in embryos and adult tissues of the sea urchin.

Authors:  G A Galau; W H Klein; M M Davis; B J Wold; R J Britten; E H Davidson
Journal:  Cell       Date:  1976-04       Impact factor: 41.582

2.  Inhibition of ribonuclease. Efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, protein ase K and heparin using a sensitive ribonuclease assay.

Authors:  S L Mendelsohn; D A Young
Journal:  Biochim Biophys Acta       Date:  1978-07-24

3.  A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos.

Authors:  G A Galau; R J Britten; E H Davidson
Journal:  Cell       Date:  1974-05       Impact factor: 41.582

4.  Ribonucleic acid isolated by cesium chloride centrifugation.

Authors:  V Glisin; R Crkvenjakov; C Byus
Journal:  Biochemistry       Date:  1974-06-04       Impact factor: 3.162

5.  Analysis of repeating DNA sequences by reassociation.

Authors:  R J Britten; D E Graham; B R Neufeld
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes.

Authors:  R P Perry; J La Torre; D E Kelley; J R Greenberg
Journal:  Biochim Biophys Acta       Date:  1972-03-14

7.  A simple and efficient method to remove ribonuclease contamination from pancreatic deoxyribonuclease preparations.

Authors:  O Brison; P Cambon
Journal:  Anal Biochem       Date:  1976-10       Impact factor: 3.365

8.  Sequence complexity of polyadenylated RNA obtained from rat brain regions and cultured rat cells of neural origin.

Authors:  B B Kaplan; B S Schachter; H H Osterburg; J S de Vellis; C E Finch
Journal:  Biochemistry       Date:  1978-12-12       Impact factor: 3.162

9.  The ribonuclease activity of crystallized pancreatic deoxyribonuclease.

Authors:  S B Zimmerman; D Sandeen
Journal:  Anal Biochem       Date:  1966-02       Impact factor: 3.365

10.  Rat insulin genes: construction of plasmids containing the coding sequences.

Authors:  A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal:  Science       Date:  1977-06-17       Impact factor: 47.728
View more
  19 in total

1.  Different mRNAs code for dopa decarboxylase in tissues of neuronal and nonneuronal origin.

Authors:  M Krieger; F Coge; F Gros; J Thibault
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

2.  Specific transcription of a 15-kilodalton zene gene in HeLa cell extracts.

Authors:  R S Boston; B A Larkins
Journal:  Plant Mol Biol       Date:  1986-01       Impact factor: 4.076

3.  Metal-specific posttranscriptional control of human metallothionein genes.

Authors:  C Sadhu; L Gedamu
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

4.  Regional localization and characterization of a DNA segment on the long arm of chromosome 21.

Authors:  D N Cooper; S C Niemann; J R Gosden; A R Mitchell; A M Goate; G S Rajendran; D A Miller; L Lim; J Schmidtke
Journal:  Hum Genet       Date:  1987-02       Impact factor: 4.132

5.  Characterization of squid enolase mRNA: sequence analysis, tissue distribution, and axonal localization.

Authors:  J T Chun; A E Gioio; M Crispino; A Giuditta; B B Kaplan
Journal:  Neurochem Res       Date:  1995-08       Impact factor: 3.996

6.  Alterations in the relative amounts of specific mRNA species in the developing human brain in Down's syndrome.

Authors:  S A Whatley; C Hall; A N Davison; L Lim
Journal:  Biochem J       Date:  1984-05-15       Impact factor: 3.857

7.  Dual tissue-specific expression of apo-AII is directed by an upstream enhancer.

Authors:  C S Shelley; F E Baralle
Journal:  Nucleic Acids Res       Date:  1987-05-11       Impact factor: 16.971

8.  Detection of measles virus genome in blood leucocytes of patients with certain autoimmune diseases.

Authors:  O G Andjaparidze; N M Chaplygina; N N Bogomolova; I B Koptyaeva; E G Nevryaeva; R G Filimonova; I E Tareeva
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

9.  Cold-induced increases in phenylethanolamine N-methyltransferase (PNMT) mRNA are mediated by non-cholinergic mechanisms in the rat adrenal gland.

Authors:  A Baruchin; R R Vollmer; L L Miner; S L Sell; E M Stricker; B B Kaplan
Journal:  Neurochem Res       Date:  1993-07       Impact factor: 3.996

10.  The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA.

Authors:  T K Leung; M Y Rajendran; C Monfries; C Hall; L Lim
Journal:  Biochem J       Date:  1990-04-01       Impact factor: 3.857
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.