Literature DB >> 24277136

The pea lectin gene family contains only one functional gene.

P A Kaminski1, D Buffard, A D Strosberg.   

Abstract

Molecular hybridization experiments have shown that the pea genome contains four regions which hybridize with pea lectin cDNA (Kaminski, Buffard, and Strosberg, 1986. Plant Science 46, 111-116). The complete organization of the pea lectin gene family was investigated. Four partial EcoRI genomic libraries were screened with a lectin cDNA (pPS 15-50) covering the entire coding region. Four positive recombinant phages, λI 101, λI 52, λIII 51 and λIV 22, were isolated and the DNA sequences of the subclones, designated respectively PSL1, PSL2, PSL3 and PSL4, were determined. PSL2, PSL3 and PSL4 are incomplete genes; the presence of several stop codons in the correct reading frames indicate that these genes cannot code for a functional lectin protein. The sequences of PSL1 and pPS 15-50 have identical coding regions. The pea lectin gene has no intervening sequences and is flanked at its 5' region by a sequence containing an exceptionally high A+T content (73%). Eucaryotic consensus sequences such as a TATA box and a polyadenylation signal are also found in the flanking regions of the PSL1 clone.

Entities:  

Year:  1987        PMID: 24277136     DOI: 10.1007/BF00015881

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN: 0167-4412            Impact factor:   4.076


  19 in total

1.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

2.  The biosynthesis and primary structure of pea seed lectin.

Authors:  T J Higgins; P M Chandler; G Zurawski; S C Button; D Spencer
Journal:  J Biol Chem       Date:  1983-08-10       Impact factor: 5.157

3.  Sequence of a pseudogene in the legumin gene family of pea (Pisum sativum L.).

Authors:  D Bown; M Levasseur; R R Croy; D Boulter; J A Gatehouse
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

4.  Soybean leghemoglobin gene family: normal, pseudo, and truncated genes.

Authors:  N Brisson; D P Verma
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

5.  Production of pea lectin in Escherichia coli.

Authors:  M E Stubbs; J P Carver; R J Dunn
Journal:  J Biol Chem       Date:  1986-05-15       Impact factor: 5.157

6.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

7.  A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.

Authors:  R M Dale; B A McClure; J P Houchins
Journal:  Plasmid       Date:  1985-01       Impact factor: 3.466

8.  cA lectin gene insertion has the structural features of a transposable element.

Authors:  L O Vodkin; P R Rhodes; R B Goldberg
Journal:  Cell       Date:  1983-10       Impact factor: 41.582

9.  The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element.

Authors:  H Bäumlein; U Wobus; J Pustell; F C Kafatos
Journal:  Nucleic Acids Res       Date:  1986-03-25       Impact factor: 16.971

10.  Selection of AUG initiation codons differs in plants and animals.

Authors:  H A Lütcke; K C Chow; F S Mickel; K A Moss; H F Kern; G A Scheele
Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598

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  12 in total

1.  The lectin gene family of Ricinus communis: cloning of a functional ricin gene and three lectin pseudogenes.

Authors:  J W Tregear; L M Roberts
Journal:  Plant Mol Biol       Date:  1992-02       Impact factor: 4.076

2.  Predicted sequence and structure of a vegetative lectin in Pisum sativum.

Authors:  J H Pak; T Hendrickson; M S Dobres
Journal:  Plant Mol Biol       Date:  1992-03       Impact factor: 4.076

3.  Distribution of glucose/mannose-specific isolectins in pea (Pisum sativum L.) seedlings.

Authors:  C L Díaz; M Hosselet; G J Logman; E van Driessche; B J Lugtenberg; J W Kijne
Journal:  Planta       Date:  1990-07       Impact factor: 4.116

4.  The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds.

Authors:  S de Pater; K Pham; I Klitsie; J Kijne
Journal:  Plant Mol Biol       Date:  1996-11       Impact factor: 4.076

5.  Mutational analysis of the sugar-binding site of pea lectin.

Authors:  R R Van Eijsden; B S De Pater; J W Kijne
Journal:  Glycoconj J       Date:  1994-08       Impact factor: 2.916

6.  Pea (Pisum sativum L.) seed isolectins 1 and 2 and pea root lectin result from carboxypeptidase-like processing of a single gene product.

Authors:  F J Hoedemaeker; M Richardson; C L Díaz; B S de Pater; J W Kijne
Journal:  Plant Mol Biol       Date:  1994-01       Impact factor: 4.076

7.  A gene/pseudogene tandem duplication encodes a cysteine-rich protein expressed during zygote development in Chlamydomonas reinhardtii.

Authors:  G L Matters; U W Goodenough
Journal:  Mol Gen Genet       Date:  1992-03

8.  Destabilization of pea lectin by substitution of a single amino acid in a surface loop.

Authors:  F J Hoedemaeker; R R van Eijsden; C L Díaz; B S de Pater; J W Kijne
Journal:  Plant Mol Biol       Date:  1993-09       Impact factor: 4.076

9.  Sugar-binding activity of pea (Pisum sativum) lectin is essential for heterologous infection of transgenic white clover hairy roots by Rhizobium leguminosarum biovar viciae.

Authors:  R van Eijsden; C L Díaz; B S de Pater; J W Kijne
Journal:  Plant Mol Biol       Date:  1995-11       Impact factor: 4.076

10.  Identification and isolation of lectin nucleotide sequences and species relationships in the genus Lens Miller.

Authors:  I Galasso; L Lioi; C Lanave; R Bollini; F Sparvoli
Journal:  Theor Appl Genet       Date:  2003-11-20       Impact factor: 5.699

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