Literature DB >> 24272865

Preparation of a tRNA-dependent wheat germ protein-synthesizing system.

H Pfitzinger1, J H Weil, D T Pillay, P Guillemaut.   

Abstract

A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with homologous aminoacyl-tRNA synthetase preparations. Such a tRNA-dependent wheat germ protein-synthesizing system, which is easy to prepare, can be used not only to translate plant cytoplasmic mRNAs in the presence of added cytoplasmic tRNAs, but also to determine the translation activity of exogenous tRNAs from various sources in the presence of either natural or in vitro synthesized mRNAs.

Entities:  

Year:  1989        PMID： 24272865     DOI： 10.1007/BF00043207

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  2 in total

1.  [Subcellular distribution of various yeast and E. coli tRNA].

Authors:  J H Weil
Journal:  Bull Soc Chim Biol (Paris)       Date:  1969

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962
  2 in total
  5 in total

1.  Codon recognition mechanisms in plant chloroplasts.

Authors:  H Pfitzinger; J H Weil; D T Pillay; P Guillemaut
Journal:  Plant Mol Biol       Date:  1990-05       Impact factor: 4.076

2.  Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAs(Phe) which differ in their minor nucleotide content.

Authors:  H Pfitzinger; L Maréchal-Drouard; D T Pillay; J H Weil; P Guillemaut
Journal:  Plant Mol Biol       Date:  1990-06       Impact factor: 4.076

3.  Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.

Authors:  M Grimm; A Nass; C Schüll; H Beier
Journal:  Plant Mol Biol       Date:  1998-11       Impact factor: 4.076

4.  Multi-domain packing in the aminoacylatable 3' end of a plant viral RNA.

Authors:  John A Hammond; Robert P Rambo; Jeffrey S Kieft
Journal:  J Mol Biol       Date:  2010-04-14       Impact factor: 5.469

5.  Prokaryotic-style frameshifting in a plant translation system: conservation of an unusual single-tRNA slippage event.

Authors:  Sawsan Napthine; Marijana Vidakovic; Roseanne Girnary; Olivier Namy; Ian Brierley
Journal:  EMBO J       Date:  2003-08-01       Impact factor: 11.598
  5 in total

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