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Literature DB >> 2102879

Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAs(Phe) which differ in their minor nucleotide content.

H Pfitzinger¹, L Maréchal-Drouard, D T Pillay, J H Weil, P Guillemaut.

Abstract

Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNA(Phe) species, namely tRNA(Phe)1 and tRNA(Phe)2. By sequence determination, we show that tRNA(Phe)2 is identical to the previously sequenced tRNA(Phe)1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAs(Phe) vary during leaf development: in etiolated leaves the undermodified tRNA(Phe)2 only represents 15% of total chloroplast tRNA(Phe), during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves.

Entities: Chemical Gene Species

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Year: 1990 PMID： 2102879 DOI： 10.1007/bf00019393

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

13 in total

1. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.

Authors: M Silberklang; A M Gillum; U L RajBhandary
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2. Preparation of a tRNA-dependent wheat germ protein-synthesizing system.

Authors: H Pfitzinger; J H Weil; D T Pillay; P Guillemaut
Journal: Plant Mol Biol Date: 1989-03 Impact factor: 4.076

3. Sequences of two bean mitochondria tRNAs(Tyr) which differ in the level of post-transcriptional modification and have a prokaryotic-like large extra-loop.

Authors: L Maréchal; P Guillemaut; J H Weil
Journal: Plant Mol Biol Date: 1985-11 Impact factor: 4.076

9. Post-transcriptional modifications of the anticodon loop region: alterations in isoaccepting species of tRNA's during development in Bacillus subtilis.

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Journal: J Bacteriol Date: 1978-07 Impact factor: 3.490

3. In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?

Authors: I Small; L Maréchal-Drouard; J Masson; G Pelletier; A Cosset; J H Weil; A Dietrich
Journal: EMBO J Date: 1992-04 Impact factor: 11.598

3 in total

Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAs(Phe) which differ in their minor nucleotide content.

1. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.

2. Preparation of a tRNA-dependent wheat germ protein-synthesizing system.

3. Sequences of two bean mitochondria tRNAs(Tyr) which differ in the level of post-transcriptional modification and have a prokaryotic-like large extra-loop.

4. Primary structure of bean chloroplastic tRNAPhe. Comparison with Euglena chloroplastic tRNAPhe.

5. Size, conformation and purity of chloroplast DNA of some higher plants.

6. The first nucleotide sequence of an organelle transfer RNA: chloroplastic tRNAphe.

7. The use of thin acrylamide gels for DNA sequencing.

8. Nucleotide sequences of three soybean chloroplast tRNAsLeu and re-examination of bean chloroplast tRNA2Leu sequence.

9. Post-transcriptional modifications of the anticodon loop region: alterations in isoaccepting species of tRNA's during development in Bacillus subtilis.

10. Changes in the content of modified nucleotides of total transfer RNA of wheat seedlings during greening.

1. Chloroplast tRNA(Asp): nucleotide sequence and variation of in vivo levels during plastid maturation.

2. New nucleotide sequence data on the EMBL File Server.

3. In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?