| Literature DB >> 24262844 |
Yingying Meng1, Changhong Yao, Song Xue, Haibo Yang.
Abstract
Fourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics of I. zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell.Entities:
Keywords: +N; Cell composition; DW; FT-IR; Fourier transform infrared spectroscopy; GL; Microalgae; NL; PAR; PL; PS II; Physiological status; Strain screening; TAG; TL; dry weight; glycolipid; neutral lipid; nitrogen deprivation; nitrogen repletion; phospholipid; photosynthetic active radiation; photosystem II; total lipid; triacylglycerol; −N
Mesh：
Substances：
Year: 2013 PMID： 24262844 DOI： 10.1016/j.biortech.2013.10.064
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642