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Literature DB >> 24258767

Flow-cytometric characterization and sorting of plant chromosomes.

Abstract

Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.

Entities: Species

Year: 1984 PMID： 24258767 DOI： 10.1007/BF00263414

Source DB: PubMed Journal: Theor Appl Genet ISSN： 0040-5752 Impact factor: 5.699

9 in total

1. Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray.

Authors: P A Werry; K M Stoffelsen
Journal: Theor Appl Genet Date: 1978-07 Impact factor: 5.699

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Authors: G Hadlaczky; G Bisztray; T Praznovszky; D Dudits
Journal: Planta Date: 1983-04 Impact factor: 4.116

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Authors: W A Bonner; H R Hulett; R G Sweet; L A Herzenberg
Journal: Rev Sci Instrum Date: 1972-03 Impact factor: 1.523

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Authors: A V Carrano; J W Gray; R G Langlois; K J Burkhart-Schultz; M A Van Dilla
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

Review 5. Chromosome mediated gene transfer.

Authors: L A Klobutcher; F H Ruddle
Journal: Annu Rev Biochem Date: 1981 Impact factor: 23.643

6. Flow microfluorometric analysis of sperm DNA content: effect of cell shape on the fluorescence distribution.

Authors: B L Gledhill; S Lake; L L Steinmetz; J W Gray; P N Dean; M A Van Dilla
Journal: J Cell Physiol Date: 1976-03 Impact factor: 6.384

7. Uptake of isolated plant chromosomes by plant protoplasts.

Authors: L Szabados; G Hadlaczky; D Dudits
Journal: Planta Date: 1981-02 Impact factor: 4.116

8. Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry.

Authors: K E Davies; B D Young; R G Elles; M E Hill; R Williamson
Journal: Nature Date: 1981-10-01 Impact factor: 49.962

9. STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES.

Authors: A BENDICH; E BORENFREUND; D J HUTCHISON
Journal: J Cell Biol Date: 1963-10 Impact factor: 10.539

9 in total

29 in total

1. Introduction: chromosomes go with the flow.

Authors: Jaroslav Dolezel; Nigel Carter; Malcolm Ferguson-Smith
Journal: Chromosome Res Date: 2004 Impact factor: 5.239

Review 2. Flow cytogenetics and plant genome mapping.

Authors: Jaroslav Dolezel; Marie Kubaláková; Jan Bartos; Jirí Macas
Journal: Chromosome Res Date: 2004 Impact factor: 5.239

3. Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells.

Authors: P K Saxena; M Mii; W L Crosby; L C Fowke; J King
Journal: Planta Date: 1986-05 Impact factor: 4.116

4. Monoparametric models of flow cytometric karyotypes with spreadsheet software.

Authors: J Conia; P Muller; S Brown; C Bergounioux; P Gadal
Journal: Theor Appl Genet Date: 1989-02 Impact factor: 5.699

5. Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips.

Authors: G Gualberti; J Doležel; J Macas; S Lucretti
Journal: Theor Appl Genet Date: 1996-05 Impact factor: 5.699

6. Stability and expression of transferred DNA in F1 tobacco transformants studied at various states of differentiation.

Authors: R Peerbolte; M Floor; P Ruigrok; J H Hoge; G J Wullems; R A Schilperoort
Journal: Planta Date: 1987-12 Impact factor: 4.116

7. Efficient induction by amiprophos-methyl and flow-cytometric sorting of micronuclei in Nicotiana plumbaginifolia.

Authors: A M de Laat; H A Verhoeven; K Sree Ramulu; P Dijkhuis
Journal: Planta Date: 1987-12 Impact factor: 4.116

8. A comparison of the effects of various spindle toxins on metaphase arrest and formation of micronuclei in cell-suspension cultures ofNicotiana plumbaginifolia.

Authors: H A Verhoeven; K Sree Ramulu; P Dijkhuis
Journal: Planta Date: 1990-10 Impact factor: 4.116

9. Preparation and flow cytometric analysis of metaphase chromosomes of tomato.

Authors: K Arumuganathan; J P Slattery; S D Tanksley; E D Earle
Journal: Theor Appl Genet Date: 1991-07 Impact factor: 5.699

10. Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy.

Authors: Channarong Sartsanga; Rinyaporn Phengchat; Kiichi Fukui; Toshiyuki Wako; Nobuko Ohmido
Journal: Chromosome Res Date: 2021-02-22 Impact factor: 5.239