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Literature DB >> 14069802

STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES.

A BENDICH, E BORENFREUND, D J HUTCHISON.

Abstract

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted and the chromosomes isolated by a series of differential centrifugations in sucrose. The isolated chromosomes were well preserved, as judged by morphological criteria. The effect of various enzymes and chemical agents on the isolated chromosomes was studied. Chymotrypsin, trypsin, and deoxyribonuclease caused a marked disintegration of the chromosomes, whereas treatment with pepsin and ribonuclease induced no significant morphological alterations.

Entities: CellLine Chemical Disease Species

Keywords: CENTRIFUGATION; CHROMOSOMES; CHYMOTRYPSIN; COLCHICINE; DEOXYRIBONUCLEASE; EXPERIMENTAL LAB STUDY; LEUKEMIA, EXPERIMENTAL; LYMPHOCYTES; MICE; PEPSIN; PHARMACOLOGY; RIBONUCLEASE; TRYPSIN

Mesh：

Substances：

Year: 1963 PMID： 14069802 PMCID： PMC2106868 DOI： 10.1083/jcb.19.1.59

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

11 in total

1. SELECTION OF CHROMOSOMAL VARIANT IN AMETHOPTERIN-RESISTANT SUBLINES OF LEUKEMIA L1210 WITH INCREASED LEVELS OF DIHYDROFOLATE REDUCTASE.

Authors: J L BIEDLER; A W SCHRECKER; D J HUTCHISON
Journal: J Natl Cancer Inst Date: 1963-09 Impact factor: 13.506

STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES.

1. SELECTION OF CHROMOSOMAL VARIANT IN AMETHOPTERIN-RESISTANT SUBLINES OF LEUKEMIA L1210 WITH INCREASED LEVELS OF DIHYDROFOLATE REDUCTASE.

2. The protective effect of spermine and other polyamines against heat denaturation of deoxyribonucleic acid.

3. Chromosome fibers from an interphase nucleus.

4. Ultracentrifugal studies on ribonucleoprotein from rat liver microsomes.

5. The stabilization of Bacillus subtilis transforming principle by spermine.

6. Action of deoxyribonuclease on lampbrush chromosomes.

7. THE PARTICULATE ORGANIZATION OF THE CHROMOSOME.

8. Chromatin threads from cell nuclei.

9. Enzymatic degradation of ribonucleoproteins of chromosomes, nucleoli and cytoplasm.

10. Experiments with chromosomes isolated from intermitotic nuclei.

1. Flow-cytometric characterization and sorting of plant chromosomes.

2. Electron-microscopic evidence for reactions of axial filaments of Leptospira with IgM and IgG antibodies.

3. Isolation and characterization of human metaphase chromosomes.

4. Isolation and partial characterization of metaphase chromosomes of a mouse ascites tumor.

5. CT banding of human chromosomes. The role of cations in the alkaline pretreatment.

6. [On the effect of nucleases and proteases on the morphology of human chromosomes].

7. Electron microscopic observations on interband fibrils in Drosophila salivary chromosomes.

Review 8. Hypoxanthine-guanine phosphoribosyl transferase deficiency.

9. Structure of metaphase chromosomes: a role for effects of macromolecular crowding.

10. A study of newt mitotic chromosomes by negative staining.