Literature DB >> 24248044

A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus.

T L Barsby1, S A Yarrow, J F Shepard.   

Abstract

Protoplasts of several spring and winter varieties of Brassica napus were isolated from hypocotyl tissue. Protoplasts divided and formed cell colonies at high frequency, without browning when cultured in modified Shepards' medium. This high efficiency of proliferation was sustained through to plant regeneration with all varieties cultured. This has been attributed to the incorporation of a reservoir medium, the presence of 2,4-D in the proliferation medium, and the presence of kinetin in conjunction with lowering of the sucrose concentration in the regeneration medium.

Entities:  

Year:  1986        PMID： 24248044     DOI： 10.1007/BF00269244

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  4 in total

1.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

2.  A simple culture method for Brassica hypototyl protoplasts.

Authors:  P V Chuong; K P Pauls; W D Beversdorf
Journal:  Plant Cell Rep       Date:  1985-02       Impact factor: 4.570

3.  Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.

Authors:  L C Li; H W Kohlenbach
Journal:  Plant Cell Rep       Date:  1982-10       Impact factor: 4.570

4.  Mesophyll cell protoplasts of potato: isolation, proliferation, and plant regeneration.

Authors:  J F Shepard; R E Totten
Journal:  Plant Physiol       Date:  1977-08       Impact factor: 8.340
  4 in total
  14 in total

1.  The combination of polima cms and cytoplasmic triazine resistance in Brassica napus.

Authors:  T L Barsby; P V Chuong; S A Yarrow; S C Wu; M Coumans; R J Kemble; A D Powell; W D Beversdorf; K P Pauls
Journal:  Theor Appl Genet       Date:  1987-04       Impact factor: 5.699

2.  Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

Authors:  S R Sikdar; G Chatterjee; S Das; S K Sen
Journal:  Plant Cell Rep       Date:  1987-12       Impact factor: 4.570

3.  Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck).

Authors:  H M Kao; W A Keller; S Gleddie; G G Brown
Journal:  Plant Cell Rep       Date:  1990-10       Impact factor: 4.570

4.  Studies on plant regeneration from cotyledonary protoplasts in Brassica campestris.

Authors:  K N Zhao; M I Whitecross; D J Bittisnich
Journal:  Plant Cell Rep       Date:  1994-01       Impact factor: 4.570

5.  An extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (Brassica oleracea var. botrytis).

Authors:  N Delpierre; J Boccon-Gibod
Journal:  Plant Cell Rep       Date:  1992-07       Impact factor: 4.570

6.  Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L.

Authors:  J E Thomzik; R Hain
Journal:  Theor Appl Genet       Date:  1988-08       Impact factor: 5.699

7.  The transfer of 'Polima' cytoplasmic male sterility from oilseed rape (Brassica napus) to broccoli (B. oleracea) by protoplast fusion.

Authors:  S A Yarrow; L A Burnett; R P Wildeman; R J Kemble
Journal:  Plant Cell Rep       Date:  1990-08       Impact factor: 4.570

8.  A simple, versatile feeder layer system for Brassica oleracea protoplast culture.

Authors:  T W Walters; E D Earle
Journal:  Plant Cell Rep       Date:  1990-10       Impact factor: 4.570

9.  Plant regeneration from hypocotyl-derived protoplasts of Brassica juncea (L.) Czern and Coss.

Authors:  P B Kirti; V L Chopra
Journal:  Plant Cell Rep       Date:  1989-03       Impact factor: 4.570

10.  The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts.

Authors:  E B Swanson; M P Coumans; G L Brown; J D Patel; W D Beversdorf
Journal:  Plant Cell Rep       Date:  1988-03       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.