Literature DB >> 24226215

Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines.

T Schmidt1, H Junghans, M Metzlaff.   

Abstract

Beta procumbens-specific DNA probes have been constructed by cloning digested total DNA in E. coli and screening the resulting recombinant plasmids in dot blot hybridizations with labelled B. procumbens and B. vulgaris DNA. Four clones (pTS1-4) have been analyzed in detail determining their degree of specificity and DNA sequence. Two clones (pTS1 and pTS2) with the highest degree of B. procumbens specificity were adapted for the squash dot hybridization with the aim of screening large numbers of individual hybrid plants (B. vulgaris x B. procumbens) carrying an alien B. procumbens chromosome (2n = 19). These addition lines carry in some cases B. procumbens resistance genes to the beet cyst nematode (Heterodea schachtii Schm.).

Entities:  

Year:  1990        PMID： 24226215     DOI： 10.1007/BF00225948

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  12 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A rapid screening technique for the detection of repeated DNA sequences in plant tissues.

Authors:  J Hutchinson; A Abbott; M O Dell; R B Flavell
Journal:  Theor Appl Genet       Date:  1985-05       Impact factor: 5.699

3.  A molecular description of telometic heterochromatin in secale species.

Authors:  J R Bedbrook; J Jones; M O'Dell; R D Thompson; R B Flavell
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

4.  Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.

Authors:  U Raeder; P Broda
Journal:  Curr Genet       Date:  1984-09       Impact factor: 3.886

5.  Isolation and characterisation of a tandemly repeated DNA sequence from the crucifer Sisymbrium irio.

Authors:  F Grellet; D Tremousaygue; M Delseny
Journal:  Indian J Biochem Biophys       Date:  1988-12       Impact factor: 1.918

6.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

7.  Genome size and the proportion of repeated nucleotide sequence DNA in plants.

Authors:  R B Flavell; M D Bennett; J B Smith; D B Smith
Journal:  Biochem Genet       Date:  1974-10       Impact factor: 1.890

8.  Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of Beta procumbens : 1. Identification of the alien chromosomes with the help of isozyme markers.

Authors:  J P Van Geyt; M Oléo; W Lange; T S De Bock
Journal:  Theor Appl Genet       Date:  1988-10       Impact factor: 5.699

9.  Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test.

Authors:  H Junghans; M Metzlaff
Journal:  Theor Appl Genet       Date:  1988-11       Impact factor: 5.699

10.  Wheat specific repetitive DNA sequences - construction and characterization of four different genomic clones.

Authors:  M Metzlaff; W Troebner; F Baldauf; R Schlegel; J Cullum
Journal:  Theor Appl Genet       Date:  1986-03       Impact factor: 5.699
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  22 in total

1.  GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines.

Authors:  Youping Wang; Karin Sonntag; Eicke Rudloff; Peter Wehling; Rod J Snowdon
Journal:  Plant Cell Rep       Date:  2005-09-08       Impact factor: 4.570

2.  Selection of monosomic addition plants in offspring families using repetitive DNA probes in Beta L.

Authors:  M Mesbah; T S De Bock; J M Sandbrink; R M Klein-Lankhorst; W Lange
Journal:  Theor Appl Genet       Date:  1996-05       Impact factor: 5.699

3.  Two different satellite DNAs in Beta vulgaris L.: evolution, quantification and distribution in the genus.

Authors:  S Santoni; A Bervillé
Journal:  Theor Appl Genet       Date:  1992-09       Impact factor: 5.699

4.  Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1 (pat-1.).

Authors:  R M Klein-Lankhorst; E M Salentijn; W G Dirkse; M Arens-de Reuver; W J Stiekema
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

5.  Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens.

Authors:  E M Salentijn; N N Sandal; R Klein-Lankhorst; W Lange; T S De Bock; K A Marcker; W J Stiekema
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

6.  High-resolution mapping of repetitive DNA by in situ hybridization: molecular and chromosomal features of prominent dispersed and discretely localized DNA families from the wild beet species Beta procumbens.

Authors:  T Schmidt; J S Heslop-Harrison
Journal:  Plant Mol Biol       Date:  1996-03       Impact factor: 4.076

7.  Distribution and evolution of two satellite DNAs in the genus Beta.

Authors:  T Schmidt; C Jung; M Metzlaff
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

8.  A BAC library of Beta vulgaris L. for the targeted isolation of centromeric DNA and molecular cytogenetics of Beta species.

Authors:  Gunnar Jacobs; Daryna Dechyeva; Torsten Wenke; Beatrice Weber; Thomas Schmidt
Journal:  Genetica       Date:  2008-04-04       Impact factor: 1.082

9.  Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses.

Authors:  Jin-Feng Chen; Xiang-Dong Luo; Chun-Tao Qian; Molly M Jahn; Jack E Staub; Fei-Yun Zhuang; Qun-Feng Lou; Gang Ren
Journal:  Theor Appl Genet       Date:  2003-12-10       Impact factor: 5.699

10.  Molecular cytogenetic mapping of chromosomal fragments and immunostaining of kinetochore proteins in Beta.

Authors:  Daryna Dechyeva; Thomas Schmidt
Journal:  Int J Plant Genomics       Date:  2009-11-08
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