| Literature DB >> 24248388 |
Mingguang Lei1, Honggui La, Kun Lu, Pengcheng Wang, Daisuke Miki, Zhizhong Ren, Cheng-Guo Duan, Xingang Wang, Kai Tang, Liang Zeng, Lan Yang, Heng Zhang, Wenfeng Nie, Pan Liu, Jianping Zhou, Renyi Liu, Yingli Zhong, Dong Liu, Jian-Kang Zhu.
Abstract
DNA methylation is important for the silencing of transposons and other repetitive elements in many higher eukaryotes. However, plant and mammalian genomes have evolved to contain repetitive elements near or inside their genes. How these genes are kept from being silenced by DNA methylation is not well understood. A forward genetics screen led to the identification of the putative chromatin regulator Enhanced Downy Mildew 2 (EDM2) as a cellular antisilencing factor and regulator of genome DNA methylation patterns. EDM2 contains a composite Plant Homeo Domain that recognizes both active and repressive histone methylation marks at the intronic repeat elements in genes such as the Histone 3 lysine 9 demethylase gene Increase in BONSAI Methylation 1 (IBM1) and is necessary for maintaining the expression of these genes by promoting mRNA distal polyadenylation. Because of its role in maintaining IBM1 expression, EDM2 is required for preventing CHG methylation in the bodies of thousands of genes. Our results thus increase the understanding of antisilencing, genome methylation patterns, and regulation of alternative RNA processing by intronic heterochromatin.Entities:
Keywords: DNA methylome; alternative polyadenylation; gene body methylation; post-transcription regulation; transcriptional gene silencing
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Year: 2013 PMID： 24248388 PMCID： PMC3890799 DOI： 10.1073/pnas.1320106110
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205